Subsarcolemmal cAMP signals monitored by cyclic nucleotide gated channels in hypertrophied cardiac myocytes

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dc.contributor.author Abi-Gerges, Aniella
dc.contributor.author Fischmeister, Rodolphe
dc.contributor.author Vandecasteele, Gregoire
dc.date.accessioned 2018-12-19T14:13:58Z
dc.date.available 2018-12-19T14:13:58Z
dc.date.copyright 2005 en_US
dc.date.issued 2018-12-19
dc.identifier.issn 0022-2828 en_US
dc.identifier.uri http://hdl.handle.net/10725/9893
dc.description.abstract The second messenger cAMP is the most important modulator of sympathetic control over cardiac contractility. Recent studies have documented the existence of local pools of cAMP in cardiac cells (Jurevicius & Fischmeister 1996, PNAS 93:295-299; Zaccolo & Pozzan, 2002 Science 295:1711-15; Rochais et al. 2004 J Biol Chem. 279:52095- 105) thought to permit selective activation of cAMP targets. In cardiac hypertrophy and failure, alterations in some of the major components of the cAMP pathway occur, but whether these modifications affect cAMP compartmentation is unknown. In an attempt to address this question, 3 weeks-old rats were subjected to aortic coarctation (H) or sham operated (S). 5 weeks after surgery, the mass of the left ventricle to tibia length ratio was 59% increased in the H group compared to the S group while the right ventricle mass to tibia length ratio was unchanged, attesting compensated hypertrophy of the heart in H rats. Isolated left ventricular myocytes from both groups were infected with an adenovirus encoding a mutant of the olfactory cyclic nucleotide-gated (CNG) channels D subunit (E583M C460W CNGA2). This channel is gated by cAMP and thus allow to follow cAMP dynamics by recording the associated cationic current (ICNG) with the whole cell patch clamp technique 24 h after infection. Cumulative concentrationresponse curve to the non-selective E-adrenergic agonist isoprenaline (Iso) indicated a 33% decrease of the maximal cAMP accumulation produced by Iso while the effect of the hydrosoluble forskolin analog, L-858051 at a saturating concentration (100 µM) was unchanged between the two groups. These results show that CNG channels allow to measure cAMP variations at the membrane in living cardiomyocytes and to detect alterations of cAMP signals during early hypertrophy. en_US
dc.language.iso en en_US
dc.title Subsarcolemmal cAMP signals monitored by cyclic nucleotide gated channels in hypertrophied cardiac myocytes en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 201402416 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Molecular and Cellular Cardiology en_US
dc.journal.volume 39 en_US
dc.article.pages 191 en_US
dc.identifier.ctation Abi-Gerges, A., Fischmeister, R., & Vandecasteele, G. (2005). Subsarcolemmal cAMP signals monitored by cyclic nucleotide gated channels in hypertrophied cardiac myocytes. Journal of Molecular and Cellular Cardiology 39, 191. en_US
dc.author.email aniella.abigerges@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.researchgate.net/publication/295966117_Subsarcolemmal_cAMP_signals_monitored_by_cyclic_nucleotide_gated_channels_in_hypertrophied_cardiac_myocytes en_US
dc.orcid.id https://orcid.org/0000-0001-9974-4023 en_US
dc.author.affiliation Lebanese American University en_US

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