Antibiotic resistance patterns and characterization of class I integron in uropathogenic escherichia coli in Lebanon. (c2008)

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dc.contributor.author Farah, Maya J.
dc.date.accessioned 2011-10-28T10:52:24Z
dc.date.available 2011-10-28T10:52:24Z
dc.date.copyright 2008 en_US
dc.date.issued 2011-10-28
dc.date.submitted 2008-02-12
dc.identifier.uri http://hdl.handle.net/10725/944
dc.description Bibliography: leaves. 52-67. en_US
dc.description.abstract Improper monitoring of antibiotic usage has hastened the development of antimicrobial resistance among uropathogenic Escherichia coli (UPEC). The rapid dissemination of antibiotic-resistance is mediated by gene transfer mechanisms involving mobile genetic elements including the recently characterized gene cassettes in integrons. Integrons are conserved DNA sequences associated with multi-drug resistance. In this study the phenotypic resistance profiles and minimum inhibitory concentration of 18 currently used antibiotics were determined using the E-test on 50 UPEC isolates collected from two Lebanese hospitals. The isolates were additionally screened using real-time PCR. for the presence of the class I integron. The variable region (VR) was then amplified in plasmids extracted from isolates that carried the integrase gene. The majority of the isolates tested were found to be resistant to cephalothin (86%), ampicillin (72%) and amoxicillin-clavulinic acid (70%), while none were resistant to fosfomycin and only 2% to imipenem. Out of the 50 tested isolates, 26% of the isolates were resistant to ten or more of the tested antibiotics, while 48% harboured the class I integron integrase gene. Within the 24 class I integron positive isolates, 18 were positive for the plasmid VR with a size ranging from 0.75 kbp to 2.5 kbp. Interestingly, 8% of the isolates that were resistant to more than ten antibiotics were negative for the class I integron. Those isolates either carried an integron other than class I or had the genes dispersed in the genome. Isolates that were positive for the presence of the class I integron but gave a negative result in the plasmid VR PCR (n=6), had the VR PCR repeated on the whole DNA extract. None of those gave a positive result; indicating that the integron might be empty. This study represents the first report from Lebanon and the Arab world that characterizes the presence of class I integron in uropathogenic E. coli isolates, and provides basal information for future pursuit and comparison especially with respect to epidemiologic distribution, antimicrobial resistance and evolution of these important pathogens. en_US
dc.language.iso en en_US
dc.subject Escherichia coli en_US
dc.subject Drug resistance in microorganisms en_US
dc.subject Genitourinary organs -- Diseases en_US
dc.title Antibiotic resistance patterns and characterization of class I integron in uropathogenic escherichia coli in Lebanon. (c2008) en_US
dc.type Thesis en_US
dc.term.submitted Fall en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school Arts and Sciences en_US
dc.author.idnumber 200103699 en_US
dc.author.commembers Dr. Fuad Hashwa
dc.author.commembers Dr. Roy Khalaf
dc.author.woa OA en_US
dc.description.physdesc 1 bound copy: viii, iii, 67 leaves; ill., tables (some col.); 31 cm. Available at RNL. en_US
dc.author.division Biology en_US
dc.author.advisor Dr. Sima Tokajian
dc.identifier.doi https://doi.org/10.26756/th.2008.44 en_US
dc.publisher.institution Lebanese American University en_US

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