Methionine supplementation enhances learning and rescues depression through epigenetic regulation of hippocampal Bdnf. (c2018)

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dc.contributor.author Ibrahim, Pascal
dc.date.accessioned 2018-10-17T07:33:52Z
dc.date.available 2018-10-17T07:33:52Z
dc.date.copyright 2018 en_US
dc.date.issued 2018-10-17
dc.date.submitted 2018-07-23
dc.identifier.uri http://hdl.handle.net/10725/8651
dc.description.abstract Brain-derived neurotrophic factor (BDNF) is essential for synaptic plasticity and learning and memory formation. It has been previously shown that the expression of BDNF is epigenetically regulated through histone methylation. Methionine, an amino acid obtained from the diet, is the precursor of S-adenosylmethionine (SAM), which is the major methyl donor in most methylation reactions. In this study, the effect of methionine supplementation on BDNF expression was assessed. Methionine treatment (100 μM) induced BDNF promoter I (pI) and coding mRNA expression in primary neuronal cell cultures. Treatment also activated Tropomyosin-related Kinase B (TrkB) signaling. Learning and memory were assessed using the Morris Water Maze. 6-Week-old C57BL/6 male mice intraperitoneally injected with a combination of 1.55175 mg/Kg methionine and 80 μg/Kg folic acid (FA), which allows regeneration of methionine, exhibited improved learning. Additionally, mice treated with methionine showed enhanced short-term memory. Inhibition of TrkB signaling led to a loss of this positive effect. These results suggest that methionine intake could enhance learning and memory possibly through modifying methylation marks in the brain and that this enhancement is mediated through BDNF, which is upregulated upon methionine treatment. en_US
dc.language.iso en en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.subject Methionine -- Therapeutic use en_US
dc.subject Learning -- Physiological aspects en_US
dc.subject Memory -- Physiological aspects en_US
dc.title Methionine supplementation enhances learning and rescues depression through epigenetic regulation of hippocampal Bdnf. (c2018) en_US
dc.type Thesis en_US
dc.term.submitted Summer en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201302355 en_US
dc.author.commembers Khalaf, Roy
dc.author.commembers Stephan, Joseph
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xi, 45 leaves; 30 cm. available at RNL. en_US
dc.author.advisor Sleiman, Sama
dc.keywords Methionine en_US
dc.keywords Methylation en_US
dc.keywords BDNF en_US
dc.keywords Learning en_US
dc.keywords Memory en_US
dc.keywords Folic acid en_US
dc.keywords High Protein Diet en_US
dc.description.bibliographiccitations Bibliography : leaves 38-45. en_US
dc.identifier.doi https://doi.org/10.26756/th.2018.102 en_US
dc.author.email pascale.ibrahim@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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