Phenotypic and proteomic characterization of Dbr48, a candida albicans cell wall protein. (c2018)

LAUR Repository

Show simple item record

dc.contributor.author Salameh, Carell C.
dc.date.accessioned 2018-10-15T08:17:07Z
dc.date.available 2018-10-15T08:17:07Z
dc.date.copyright 2018 en_US
dc.date.issued 2018-10-15
dc.date.submitted 2018-08-08
dc.identifier.uri http://hdl.handle.net/10725/8628
dc.description.abstract The fungus Candida albicans is an opportunistic pathogen that possesses multiple factors and mechanisms that control virulence. The most important virulence factors and antigenic determinants are the cell wall proteins as they are the first elements contacting the host. Ddr48 is a cell wall protein consisting of 212 amino acids. This protein had been previously studied where it was found to be required for tolerance to hydrogen peroxide oxidative stress, drug resistance, filamentation and virulence. DDR48 was found to be haploid insufficient and essential as only a heterozygous strain was generated and not a homozygous null strain. In this study, we aimed to further elucidate the role of Ddr48 by subjecting our DDR48 heterozygous strain to a battery of assays in order to achieve additional phenotypic characterization. In addition, a proteomic approach was utilized to determine differentially expressed cell wall proteins detected exclusively in the wild type strain. Our results showed that the heterozygous mutant strain exhibited a 10% decrease in adhesion mirrored by a 20% decrease in biofilm formation, and a slight sensitivity to oxidative stress agents and SDS. Both strains showed similar hyphae formation ability, temperature tolerance, calcofluor white and Congo red sensitivities, chitin content, and virulence. For proteomic characterization, a total of 8 and 10 proteins were identified exclusively in the wild type strain grown under filamentous and non-filamentous conditions respectively. These proteins included members of the superoxide dismutase family required for resisting superoxide stress such as Sod4 and Sod6. Additionally, proteins responsible for adhesion (Als3, Hyr4, Pmt1, and Utr2), biofilm formation (Hsp90, Ece1, Rim9, Ipp1, and Pra1) and cell wall integrity (Utr2 and Pga4) were found. The lack of detection of these proteins in the heterozygous mutant strain go a long way in explaining the observed phenotypes. en_US
dc.language.iso en en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.subject Bacterial cell walls en_US
dc.subject Candida albicans en_US
dc.title Phenotypic and proteomic characterization of Dbr48, a candida albicans cell wall protein. (c2018) en_US
dc.type Thesis en_US
dc.term.submitted Summer en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 200602364 en_US
dc.author.commembers Tokajian, Sima
dc.author.commembers Stephan, Joseph
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xii, 64 leaves; ill.; 30 cm. available at RNL. en_US
dc.author.advisor Khalaf, Roy
dc.keywords Candida albicans en_US
dc.keywords Cell Wall en_US
dc.keywords Ddr48 en_US
dc.keywords Adhesion en_US
dc.keywords Biofilm en_US
dc.keywords Oxidative Stress en_US
dc.keywords Proteomics en_US
dc.keywords Tandem Mass Spectrometry en_US
dc.description.bibliographiccitations Bibliography : leaves 50-61. en_US
dc.identifier.doi https://doi.org/10.26756/th.2018.90 en_US
dc.author.email carell.salameh@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search LAUR

Advanced Search


My Account