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Human recombinant arginase I (Co)-PRG5000 [HuArgl (Co)-PRG5000]- induced arginine depletion selectively inhibits colon cancer cell migration and invasion. (c2018)

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dc.contributor.author Koussa, Houssam Khaled Al
dc.date.accessioned 2018-10-02T07:47:42Z
dc.date.available 2018-10-02T07:47:42Z
dc.date.copyright 2018 en_US
dc.date.submitted 2018-05-23
dc.identifier.uri http://hdl.handle.net/10725/8537
dc.description.abstract Many arginine deprivation studies have been done on different cancer cell lines to understand the complete mechanisms of HuArgI(Co)-PEG5000. Colorectal cancer is the third most common type of cancer worldwide, and it represents over half of all gastrointestinal cancer death. Therefore, the first purpose of this study is to examine the cytotoxic effect of HuArgI(Co)-PEG5000 on colorectal cancer cell lines (HT-29, Caco-2, Sw837, Sw1116, SKco-1). The second aim is to investigate the effect of arginase depletion on colorectal cancer cell line Caco-2 metastatic and invasive abilities. This is achieved by performing cytotoxicity, 2D and 3D migration assays, western immunoblotting, immunostaining, and Förster Resonance energy transfer. Analysis of the results show that HuArgI(Co)-PEG5000 downregulates ASS1 and RhoA expression levels while it also downregulates cell migration, adhesion, and invasion in Caco-2 cell lines. However, L-citrulline can significantly restore arginine levels and hence counter the effect of HuArgI(Co)-PEG5000. Therefore, we can conclude that colorectal cancer is partial auxotrophic to arginine depletion and that arginine depletion plays an important role in regulating cancer cells motility and invasion. en_US
dc.language.iso en en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.subject Cancer cells -- Growth -- Regulation en_US
dc.subject Colon (Anatomy) -- Cancer en_US
dc.subject Rectum -- Cancer en_US
dc.subject Arginine en_US
dc.title Human recombinant arginase I (Co)-PRG5000 [HuArgl (Co)-PRG5000]- induced arginine depletion selectively inhibits colon cancer cell migration and invasion. (c2018) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201101023 en_US
dc.author.commembers Nawas, Tarek
dc.author.commembers Khalaf, Roy
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xiii, 60 leaves; col. ill.; 30 cm. available at RNL. en_US
dc.author.advisor El Sibai, Mirvat
dc.author.advisor Abi-Habib, Ralph
dc.keywords Colorectal cancer en_US
dc.keywords HuArgI(Co)-PEG5000 en_US
dc.keywords L-citrulline en_US
dc.keywords RhoA en_US
dc.keywords Focal adhesion en_US
dc.keywords metalloproteinase en_US
dc.description.bibliographiccitations Bibliography : leaves 52-60. en_US
dc.identifier.doi https://doi.org/10.26756/th.2018.69 en_US
dc.author.email houssam.alkoussa@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US


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