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Epidemiological investigation of a foodborne outbreak in Lebanon caused by vibrio parahaemolyticus. (c2018)

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dc.contributor.author Fattel, Leila F.
dc.date.accessioned 2018-10-02T05:32:12Z
dc.date.available 2018-10-02T05:32:12Z
dc.date.copyright 2018 en_US
dc.date.submitted 2018-04-10
dc.identifier.uri http://hdl.handle.net/10725/8533
dc.description.abstract Vibrio parahaemolyticus is a halophilic, Gram-negative, marine bacterium. It is found inhabiting as part of the microbiota in aquatic animals, especially shellfish, and is non-pathogenic but can cause acute hepatopancreatic necrosis in shrimps in the presence of two toxins PirAVP and PirBVP. An unconventional V. parahaemolyticus strain has been linked to disorders in humans too. Upon consumption of contaminated seafood, the virulent strain causes gastroenteritis through hemolysins, such as tdh, trh, and tlh. V. parahaemolyticus is still generally susceptible to various groups of antibacterial drugs, however, its resistance to blactams was previously detected. A pandemic strain of V. parahaemolyticus belonging to serotype O3:K6 is responsible for most of the clinical outbreaks worldwide. The pandemic spread of this strain was correlated to the acquisition of orf8 via a bacteriophage, leading to an increase in virulence. In addition, pathogenicity islands and mobile elements contributed to the evolution of the O3:K6 serotype. In this study, V. parahaemolyticus was collected from the Middle East Institute of Health University Hospital in Lebanon. The isolates were analyzed and compared using whole-genome sequencing. Virulence was tested by the detection of hemolysins tdh, trh, and tlh. Resistance was analyzed by antibiotic susceptibility testing and the performance of PCRs for b-lactamases, and quinolone resistance determinants. Genetic relatedness of the isolates was studied by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole genome-based SNP analysis (wgSNP). All of the isolates had the tdh+, trh-, GS-PCR+ genotype, which is a characteristic of the O3:K6 pandemic clone. The isolates were resistant to ampicillin (100%), ceftazidime (86%), ticarcillin (14%), and amikacin (14%). All of the isolates belonged to ST3 and had very similar phylogenetic fingerprints. The isolates undertaken in this study exhibited almost identical resistance, virulence, and phylogenetic patterns, indicating an outbreak linked to the spread of the pandemic O3:K6 serotype in the country. en_US
dc.language.iso en en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.subject Vibrio parahaemolyticus en_US
dc.subject Epidemics -- Lebanon en_US
dc.subject Seafood -- Contamination -- Lebanon en_US
dc.title Epidemiological investigation of a foodborne outbreak in Lebanon caused by vibrio parahaemolyticus. (c2018) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201202671 en_US
dc.author.commembers Daher, Costantine
dc.author.commembers Nawas, Tarek
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xii, 42 leaves; col. ill.; 30 cm. available at RNL. en_US
dc.author.advisor Tokajian, Sima
dc.keywords Vibrio parahaemolyticus en_US
dc.keywords Gastroenteritis en_US
dc.keywords tdh en_US
dc.keywords O3:K6 en_US
dc.keywords outbreak en_US
dc.keywords Lebanon en_US
dc.description.bibliographiccitations Bibliography : leaves 34-42. en_US
dc.identifier.doi https://doi.org/10.26756/th.2018.66 en_US
dc.author.email leila.fattel@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US


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