Molecular characterization of carbapenem resistant klebsiella pneumoniae and klebsiella quasipneumoniae isolated from Lebanon. (c2018)

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dc.contributor.author Arabaghian, Harout K.
dc.date.accessioned 2018-10-01T10:40:43Z
dc.date.available 2018-10-01T10:40:43Z
dc.date.copyright 2018 en_US
dc.date.issued 2018-10-01
dc.date.submitted 2018-04-20
dc.identifier.uri http://hdl.handle.net/10725/8532
dc.description.abstract Klebsiella pneumoniae is a Gram-negative rod, mostly known for causing nosocomial infections in the immunocompromised, the elderly, and the neonates. In the last decade, the emergence and spread of both extensively drug resistant (XDR) and hypervirulent K. pneumoniae (hvKP) clones have rendered the species a major public health concern. Whole-genome sequencing (WGS) was used for the molecular characterization of 32 carbapenem-resistant K. pneumoniae (CRKP) and two carbapenem-resistant K. quasipneumoniae. Resistance was assessed by performing antibiotic susceptibility testing (AST) and PCRs for the most common extended-spectrum β-lactamase and carbapenemase encoding genes. Virulence was studied by sequencing the wzi gene and determinants confirmed in silico. PCR-based replicon typing (PBRT) was performed to identify plasmid incompatibility groups. Genetic relatedness of the isolates was determined by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and whole-genome based SNPs analysis. All of the isolates were nonsusceptible to at least one of the three carbapenems tested, with 100% (34/34) of the isolates being non-susceptible to ertapenem, 85.3% (29/34) to imipenem, and 70.6% (24/34) to meropenem. This phenotypic resistance was found to be due to either blaOXA- 48 (61.8%; 21/34) or blaNDM-1 (14.7%; 5/34) or blaNDM-7 (2.9%; 1/34), or the coupling of ESBLs and outer membrane porin loss or modification (20.6%; 7/34). IncL and IncFIIK were the most common replicons detected (91.2%; 31/34). Twenty and 21 different capsular types (K-types) and sequence types (STs) were detected, respectively. Thus our results reveal a very high level of diversity in the K. pneumoniae population circulating in Lebanon. We also report the presence of carbapenem-resistant K. quasipneumoniae, the circulation of multi-replicon plasmids harboring NDM type enzymes, and the convergence of extensively drug resistant and hypervirulent features in Lebanon.
dc.language.iso en en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.subject Drug resistance in microorganisms en_US
dc.subject Klebsiella quasipneumoniae en_US
dc.subject Nosocomial infections -- Prevention en_US
dc.subject Klebsiella pneumoniae en_US
dc.title Molecular characterization of carbapenem resistant klebsiella pneumoniae and klebsiella quasipneumoniae isolated from Lebanon. (c2018) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201201747 en_US
dc.author.commembers Khalaf, Roy
dc.author.commembers Wex, Brigitte
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xv, 63 leaves; col. ill.; 30 cm. available at RNL. en_US
dc.author.advisor Tokajian, Sima
dc.keywords Klebsiella pneumoniae en_US
dc.keywords NDM en_US
dc.keywords Klebsiella quasipneumoniae en_US
dc.keywords carbapenems en_US
dc.keywords WGS en_US
dc.keywords SNPs en_US
dc.keywords OXA-48 en_US
dc.description.bibliographiccitations Bibliography : leaves 52-63. en_US
dc.identifier.doi https://doi.org/10.26756/th.2018.65 en_US
dc.author.email harout.arabaghian@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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