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Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence

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dc.contributor.author El Khoury, Pamela
dc.contributor.author Awad, Andy
dc.contributor.author Wex, Brigitte
dc.contributor.author Khalaf, Roy A.
dc.date.accessioned 2018-09-13T10:48:16Z
dc.date.available 2018-09-13T10:48:16Z
dc.date.copyright 2018 en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/10725/8457
dc.description.abstract We have previously characterized Pir32, a Candia albicans cell wall protein that we found to be involved in filamentation, virulence, chitin deposition, and resistance to oxidative stress. Other than defining the cell shape, the cell wall is critical for the interaction with the surrounding environment and the point of contact and interaction with the host surface. In this study, we applied tandem mass spectrometry combined with bioinformatics to investigate cell wall proteome changes in a pir32 null strain. A total of 16 and 25 proteins were identified exclusively in the null mutant strains grown under non-filamentous and filamentous conditions. These proteins included members of the PGA family with various functions, lipase and the protease involved in virulence, superoxide dismutases required for resisting oxidative stress, alongside proteins required for cell wall remodeling and synthesis such as Ssr1, Xog1, Dfg5 and Dcw1. In addition proteins needed for filamentation like Cdc42, Ssu81 and Ucf1, and other virulence proteins such as Als3, Rbt5, and Csa2 were also detected. The detection of these proteins in the mutant and their lack of detection in the wild type can explain the differential phenotypes previously observed. en_US
dc.language.iso en en_US
dc.title Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200300427 en_US
dc.author.idnumber 200603698 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Plos one en_US
dc.journal.volume 13 en_US
dc.journal.issue 3 en_US
dc.article.pages 1-16 en_US
dc.identifier.doi https://doi.org/10.1371/journal.pone.0194403 en_US
dc.identifier.ctation El Khoury, P., Awad, A., Wex, B., & Khalaf, R. A. (2018). Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence. PloS one, 13(3), 1-16. en_US
dc.author.email roy.khalaf@lau.edu.lb en_US
dc.author.email brigitte.wex@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194403 en_US
dc.author.affiliation Lebanese American University en_US


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