Preliminary evaluation of technetium-99m-labeled thymoquinone

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dc.contributor.author Elaridi, Jomana
dc.contributor.author Velho-Pereira, Reelma
dc.contributor.author Gaikwad, Rajiv V.
dc.date.accessioned 2018-08-30T11:54:46Z
dc.date.available 2018-08-30T11:54:46Z
dc.date.copyright 2017 en_US
dc.date.issued 2018-08-30
dc.identifier.issn 0975-8585 en_US
dc.identifier.uri http://hdl.handle.net/10725/8400
dc.description.abstract Thymoquinone (TQ) was radiolabeled with sodium pertechnetate (Na 99m TcO4) by reduction with stannous chloride (SnCl2). The labeling efficiency of 99m Tc-TQ was determined by iTLC. Labeling conditions were optimized for pH, amount of SnCl2 required for radiolabeling and incubation time. Specifically, direct radiolabeling of 2.5 mg/ml TQ with 250 µg/ml of stannous chloride at pH 7 with gentle mixing and incubation for 30 minutes generated the desired 99m Tc-TQ complex with 95% labeling efficiency. The in vitro stability in serum and physiologic saline showed that the radiolabeled complex was stable for 24 h. Based on the percent injected dose (%ID), the highest uptake of the 99m Tc-TQ was found in the RES post IV injection in normal mice. Oral administration of TQ led to predominant uptake of the radiolabeled complex in the stomach and the intestine. Thus, a quick, efficient and reproducible method for radiolabeling thymoquinone with the 99m Tc-radionuclide was developed and optimized. The TQ-radiotracer was evaluated for radiochemical purity, stability and tissue and organ distribution in mice. en_US
dc.language.iso en en_US
dc.title Preliminary evaluation of technetium-99m-labeled thymoquinone en_US
dc.type Article en_US
dc.description.version Published en_US
dc.title.subtitle radiotracer uptake studies using gamma scintigraphy imaging technique en_US
dc.author.school SAS en_US
dc.author.idnumber 201100947 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Research Journal of Pharmaceutical, Biological and Chemical Sciences en_US
dc.journal.volume 8 en_US
dc.journal.issue 5 en_US
dc.article.pages 603 en_US
dc.identifier.ctation Velho-Pereira, R., Jagtap, A., Gaikwad, R.,& Elaridi J. (2015). Preliminary Evaluation of Technetium-99m-Labeled Thymoquinone: Radiotracer Uptake Studies using Gamma Scintigraphy Imaging Technique. Research Journal of Pharmaceutical, Biological and Chemical Sciences 8(5): 603 en_US
dc.author.email jomana.aridi@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.researchgate.net/publication/320065449_Preliminary_Evaluation_of_Technetium-99m-Labeled_Thymoquinone_Radiotracer_Uptake_Studies_using_Gamma_Scintigraphy_Imaging_Technique en_US
dc.author.affiliation Lebanese American University en_US

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