Typing and comparative genome analysis of Brucella melitensis isolated from Lebanon

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dc.contributor.author Tokajian, Sima
dc.contributor.author Abou Zaki, Natalia
dc.contributor.author Salloum, Tamara
dc.contributor.author Osman, Marwan
dc.contributor.author Rafei, Rayane
dc.contributor.author Hamze, Monzer
dc.date.accessioned 2018-06-26T10:02:03Z
dc.date.available 2018-06-26T10:02:03Z
dc.date.copyright 2017 en_US
dc.identifier.issn 0378-1097 en_US
dc.identifier.uri http://hdl.handle.net/10725/8101
dc.description.abstract Brucella melitensis is the main causative agent of the zoonotic disease brucellosis. This study aimed at typing and characterizing genetic variation in 33 Brucella isolates recovered from patients in Lebanon. Bruce-ladder multiplex PCR and PCR-RFLP of omp31, omp2a and omp2b were performed. Sixteen representative isolates were chosen for draft-genome sequencing and analyzed to determine variations in virulence, resistance, genomic islands, prophages and insertion sequences. Comparative whole-genome single nucleotide polymorphism analysis was also performed. The isolates were confirmed to be B. melitensis. Genome analysis revealed multiple virulence determinants and efflux pumps. Genome comparisons and single nucleotide polymorphisms divided the isolates based on geographical distribution but revealed high levels of similarity between the strains. Sequence divergence in B. melitensis was mainly due to lateral gene transfer of mobile elements. This is the first report of an in-depth genomic characterization of B. melitensis in Lebanon. en_US
dc.language.iso en en_US
dc.title Typing and comparative genome analysis of Brucella melitensis isolated from Lebanon en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199736770 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal FEMS Microbiology letters en_US
dc.journal.volume 364 en_US
dc.journal.issue 19 en_US
dc.keywords Brucella melitensis en_US
dc.keywords Brucellosis en_US
dc.keywords PCR en_US
dc.keywords RFLP en_US
dc.keywords Draft-genome sequencing, en_US
dc.keywords SNPs en_US
dc.identifier.doi https://doi.org/10.1093/femsle/fnx199 en_US
dc.identifier.ctation Abou Zaki, N., Salloum, T., Osman, M., Rafei, R., Hamze, M., & Tokajian, S. (2017). Typing and comparative genome analysis of Brucella melitensis isolated from Lebanon. FEMS microbiology letters, 364(19). en_US
dc.author.email stokajian@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://academic.oup.com/femsle/article/364/19/fnx199/4157788 en_US
dc.author.affiliation Lebanese American University en_US

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