The effect of Interleukin-13 on Leishmania major-induced infection in rats. (c2005)

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dc.contributor.author Habre, Marc
dc.date.accessioned 2011-10-12T09:09:39Z
dc.date.available 2011-10-12T09:09:39Z
dc.date.copyright 2005 en_US
dc.date.issued 2011-10-12
dc.date.submitted 2005-08-29
dc.identifier.uri http://hdl.handle.net/10725/745
dc.description Bibliography: leaves 116-154. en_US
dc.description.abstract Interleukin-13 is a relatively new cytokine with continuously growmg importance to medical research and medical practice. IL-13 has been shown to be involved in suppressing the immune responses in some cases, while promoting it in others. We investigated the effects of recombinant IL-13 (rIL-13) on the host response to Leishmania mrgor in rats. IL-13 treatment started 1 hour pre-infection and was maintained daily for 8 days post-infection. Acquired resistance against L mrg'or is a typical T helper (Th) 1 dominated immune response, whereas Th2 cytokines are thought to worsen leishmaniasis. Although IL-13 has been described as a Th2 cytokine with deactivating antiinflammatory activities, we found that treatment with IL-13 increased the levels of IL-l~; a major pro-inflammatory cytokine. Consequently, IL-13 increased pain perception in rats as assessed by the hot plate tests. Furthermore, IL-13 has been reported to down-regulate IL-12; a key cytokine in the propagation of the Thl immune response, and therefore promoting a Th2 response. In contrast, we found that IL-13 increased IL-12p70 production, but had no effect on the levels of IL-4; a major Th2 cytokine. Further analysis included quantification of L. mqjor parasites from paws (infection site), liver and blood of rats using real-time peR. The tests revealed that 99.99% of the injected parasites were cleared in both IL-13 treated and non-treated rats at day 30 post-infection, suggesting that both groups followed the Thl healing pathway. en_US
dc.language.iso en en_US
dc.subject Interleukins en_US
dc.subject Interleukin-13 en_US
dc.subject Leishmaniasis, Cutaneous en_US
dc.title The effect of Interleukin-13 on Leishmania major-induced infection in rats. (c2005) en_US
dc.type Thesis en_US
dc.term.submitted Summer II en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school Arts and Sciences en_US
dc.author.idnumber 199803850 en_US
dc.author.commembers Dr. Roy Khalaf
dc.author.commembers Dr. Costantine Daher
dc.author.woa OA en_US
dc.description.physdesc 1 bound copy: xix, 154 leaves; col. ill.; 30 cm. available at RNL. en_US
dc.author.division Biology en_US
dc.author.advisor Dr. George Baroudy
dc.identifier.doi https://doi.org/10.26756/th.2005.43 en_US
dc.publisher.institution Lebanese American University en_US

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