Analysis of the pentane fraction obtained from Daucus carota oil extract and its activity against colon cancer cell lines

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dc.contributor.author Taleb, RI
dc.contributor.author Chababi, W.
dc.contributor.author El-Sibai, M.
dc.contributor.author Daher, C.
dc.contributor.author Mroueh, M.
dc.date.accessioned 2018-04-19T09:37:45Z
dc.date.available 2018-04-19T09:37:45Z
dc.date.copyright 2012 en_US
dc.date.issued 2018-04-19
dc.identifier.issn 1439-0221 en_US
dc.identifier.uri http://hdl.handle.net/10725/7427
dc.description.abstract Phytotherapy has been widely used throughout the years for the treatment of a variety of diseases such as colon and colorectal cancers. Recent studies in our laboratories have shown that Daucus carota (Linnaeus) ssp. carota possesses potential anti-tumor activity. Oil extract (1:1 methanol/acetone) of Daucus carota grown in Lebanon was subjected to a chromatographic separation using a pentane based mobile phase to yield four fractions. GCMS analysis of the first fraction, F1, reveals 11 components present in excess of 1%, such as α-longipinene (2.97%), humulene (13.2%), (10S,11S)-himachala-3(12)-4-diene (2.56%), caryophyllene (7.41%), β-selinene (32.6%), β-himachalene (3.96%) and caryophyllene oxide (2.33%). The anticancer effect of the F1 fraction was tested against colon cancer cell lines, HT-29 and Caco-2, and the results demonstrated that the F1 fraction had an anti-proliferative effect against both cell lines. The F1 fraction also exhibited dose dependent cytotoxicity against all tested cell lines with IC50 values of 25µg/mL for HT-29 cells and 15µg/mL for Caco-2 cells. A comparitive study of Daucus carota from various geographic locations including Lebanon and Europe revelead significant differences regarding the major components present in the methanol/acetone oil extract. In conclusion, the composition of the Daucus carota oil extract is dependant on geographical location and that the F1 fraction of the oil extract obtained from Daucus carota grown in Lebanon posseses anticancer activity. en_US
dc.language.iso en en_US
dc.title Analysis of the pentane fraction obtained from Daucus carota oil extract and its activity against colon cancer cell lines en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.school SOP en_US
dc.author.idnumber 200901968 en_US
dc.author.idnumber 200703859 en_US
dc.author.idnumber 199590020 en_US
dc.author.idnumber 199190130 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Planta Medica en_US
dc.journal.volume 78 en_US
dc.journal.issue 11 en_US
dc.article.pages PI146 en_US
dc.identifier.doi http://dx.doi.org/10.1055/s-0032-1320834 en_US
dc.identifier.ctation Taleb, R. I., Chababi, W., El-Sibai, M., Daher, C., & Mroueh, M. (2012). Analysis of the pentane fraction obtained from Daucus carota oil extract and its activity against colon cancer cell lines. Planta Medica, 78(11), PI146. en_US
dc.author.email robin.taleb@lau.edu.lb en_US
dc.author.email mirvat.elsibai@lau.edu.lb en_US
dc.author.email mmroueh@lau.edu.lb en_US
dc.author.email cdaher@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0032-1320834 en_US
dc.orcid.id https://orcid.org/0000-0003-4084-6759 en_US
dc.author.affiliation Lebanese American University en_US

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