The in vitro anticancer effects of himachalol from Cedrus Libani. (c2017)

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dc.contributor.author Elias, Andree M.
dc.date.accessioned 2018-03-06T11:33:08Z
dc.date.available 2018-03-06T11:33:08Z
dc.date.copyright 2017 en_US
dc.date.submitted 2017-07-27
dc.identifier.uri http://hdl.handle.net/10725/7187
dc.description.abstract Cedrus libani, a member of the genus Cedar and family Pinaceae, is frequently used in traditional Lebanese medicine to treat several ailments. Essential oils extracted from this plant were found to exhibit important biomedical properties, including anti-ulcerogenic, anti-viral, neuroprotective as well as anti-proliferative activity. The present study aims to isolate himachalol, a major compound in the wood oil extract, and assess its anticancer activity against skin (B16F-10), brain (SF-268), colorectal (Caco-2 and HT-29) and ovarian (Sk-OV-3) cancer cells. 2-himachalen-7-ol (7-HC) was isolated using silica gel column chromatography, identified using GC-MS and confirmed with NMR studies. Cytotoxicity assays were performed using WST proliferation kit. Cell cycle analysis and cell apoptosis were evaluated using flow cytometry. The level of Akt, Erk, Bax and Bcl-2 proteins were determined via western blot analysis. Data revealed that 7-HC exhibits potent anti-proliferative activities against most treated cells, with B16F-10 being the most sensitive and Sk-OV-3 the least sensitive. Flow cytometry analysis demonstrated that the cytotoxicity of 7-HC is mediated via arresting the cell at Sub-G1 and inducing cell death via late apoptosis. Western blot analysis showed that treatment with 7-HC causes a downregulation of the level of anti-apoptotic proteins (Bcl-2) and an up-regulation of the level of pro-apoptotic proteins (Bax). A decline in the level of phosphorylated Erk and Akt was also noted. In conclusion, the current study revealed that 7-HC has a remarkable anticancer activity against a panel of cancer cell lines, an effect that may be partly attributed to the inhibition of the PI3K/Akt and MAPK/Erk pathways. en_US
dc.language.iso en en_US
dc.subject Dissertations, Academic en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Cancer -- Prevention en_US
dc.subject Cedar trees -- Therapeutic use en_US
dc.subject Wood oil -- Therapeutic use en_US
dc.subject Cancer cells en_US
dc.title The in vitro anticancer effects of himachalol from Cedrus Libani. (c2017) en_US
dc.type Thesis en_US
dc.term.submitted Summer en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 200902697 en_US
dc.author.commembers El Sibai, Mirvat
dc.author.commembers Mroueh, Mohamad
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xvi, 80 leaves; 31 cm. available at RNL. en_US
dc.author.advisor Daher, Costantine
dc.keywords Cedrus libani en_US
dc.keywords 7-HC en_US
dc.keywords Apoptosis en_US
dc.keywords Cell cycle arrest en_US
dc.keywords Cancer en_US
dc.keywords MAPK/Erk en_US
dc.description.bibliographiccitations Bibliography: p. 68-80. en_US
dc.identifier.doi https://doi.org/10.26756/th.2018.38 en_US
dc.author.email andree.elias@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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