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The role of RHD1, a putative essential transmembrane protein, in filamentation, virulence and drug susceptibility. (c2008)

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dc.contributor.author Yazbeck, Pascal
dc.date.accessioned 2011-10-06T10:58:20Z
dc.date.available 2011-10-06T10:58:20Z
dc.date.copyright 2008 en_US
dc.date.issued 2011-10-06
dc.date.submitted 2008-06-13
dc.identifier.uri http://hdl.handle.net/10725/698
dc.description Includes bibliographical references (leaves 35-43). en_US
dc.description.abstract Candida albicans is one of the pathogenic fungal organisms that cause mucosal and systemic infections leading to death in immunocompromised individuals. Its pathogenicity can be ascribed to its ability to exist in different morphological states. Many transmembrane proteins have been implicated in morphological switching from yeast to hyphal form. Such is Rhdl (Repressed under Hyphal Development), a putative transmembrane protein that was found to be repressed five fold under yeast to hyphal transition. The aim of the present study is to investigate and characterize the role of Rhdl in in vitro filamentation and in vivo virulence in a mouse model through generating a homozygous null mutant strain. Moreover, this study aims to assess the antifungal drug susceptibility of mutant strain against azoles, caspofungin and anlphotericin B, using E-test method as well as oxidative stress response to a lethal dose of hydrogen peroxide and white opaque switching. Interestingly, RHDI appears to be an essential gene since only a heterozygote strain was generated. However, no haploinsufficiency was observed as both wild type and mutant showed similar behavior on different solid and liquid hyphal inducing and non inducing media. In vivo, the mice survival rate was 0% after 16 days of post injection with the heterozygote strain compared to 0% survival at 19 days with parental strain. Moreover, Rhd 1 does not appear to play a ro le in drug resistance, white opaque switching and oxidative stress. Finally, a BLASTP of the Rhdl ORF against the entire C albicans genome revealed homology to orf.l9.l464 a yet uncharacterized protein. This homology did not span the N terminal signal peptide (1 -11) or the transmembrane region (38-58). en_US
dc.language.iso en en_US
dc.subject Candida albicans en_US
dc.subject Candidiasis en_US
dc.subject Morphology en_US
dc.subject Filamentous fungi en_US
dc.subject Virulence (Microbiology) -- Molecular aspects en_US
dc.title The role of RHD1, a putative essential transmembrane protein, in filamentation, virulence and drug susceptibility. (c2008) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.school Arts and Sciences en_US
dc.author.idnumber 200503380 en_US
dc.author.commembers Dr. Sima Tokajian en_US
dc.author.commembers Dr. Brigitte Wex en_US
dc.author.woa OA en_US
dc.author.department MS in Molecular Biology en_US
dc.description.physdesc 1 bound copy: vi, 43 leaves; ill. (some col.); 31 cm. Available at RNL. en_US
dc.author.division Biology en_US
dc.author.advisor Dr. Roy Khalaf en_US
dc.identifier.doi https://doi.org/10.26756/th.2008.20


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