Simple liquid chromatographic determination of minocycline in brain and plasma

Milane, A.; Fernandez, C.; Bensimon, G.; Meininger, V.

Simple liquid chromatographic determination of minocycline in brain and plasma

Milane, A.; Fernandez, C.; Bensimon, G.; Meininger, V.

URL: https://link.springer.com/article/10.1365/s10337-006-0167-5

Date: 2017-12-20

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Abstract:

A new high-performance liquid chromatography assay was developed for the determination of minocycline in plasma and brain. A solid–liquid extraction procedure was coupled with a reversed-phase HPLC system. The system requires a mobile phase consisting of acetonitrile:water:perchloric acid (26:74:0.25, v/v/v) adjusted to pH 2.5 with 5 M sodium hydroxide for elution through a RP8 column (250 × 3.0 mm, i.d.) with UV detection set at 350 nm. The method proved to be accurate, precise (RSD < 20%) and linear between 0.15–20 μg mL−1 in plasma and 1–20 μg mg−1 in brain. The method was successfully applied to a blood-brain barrier minocycline transport study.

Citation:

Milane, A., Fernandez, C., Bensimon, G., Meininger, V., & Farinotti, R. (2007). Simple liquid chromatographic determination of minocycline in brain and plasma. Chromatographia, 65(5-6), 277-281.