Delineating melanoma using multimodal polarized light imaging

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dc.contributor.author Tannous, Zeina
dc.contributor.author Al-Arashi, Munir
dc.contributor.author Shah, Sonali
dc.contributor.author Yaroslavsky, Anan N.
dc.date.accessioned 2017-11-10T10:45:25Z
dc.date.available 2017-11-10T10:45:25Z
dc.date.copyright 2009 en_US
dc.date.issued 2017-11-10
dc.identifier.issn 1096-9101 en_US
dc.identifier.uri http://hdl.handle.net/10725/6572
dc.description.abstract Background and Significance Melanoma accounts for 3% of all skin cancers but causes 83% of skin cancer deaths. The first step in treatment of melanoma is the removal of the lesions, usually by surgical excision. Currently most lesions are removed without intraoperative margin control. Post-operative methods inspect 1–2% of the surgical margin and are prone to sampling errors. In this study we evaluate the use of reflectance and fluorescence polarization imaging for the demarcation of melanoma in thick fresh skin excisions. Materials and Methods Pigmented lesions clinically suspicious for melanoma were elliptically excised with proper margins. Elliptical surgical excisions were vertically bisected along the short axis of the specimen into two halves in the middle of the pigmented lesions. The vertically bisected tumor face was imaged. After that, one half of the sample was briefly stained in aqueous 2 mg/ml solution of tetracycline, whereas another half was stained in 0.2 mg/ml aqueous solution of methylene blue. Then both specimens were reimaged. Reflectance images were acquired in the spectral range between 390 and 750 nm. Fluorescence images of the tetracycline-stained tissue were excited at 390 nm and registered between 450 and 700 nm. Fluorescence of the methylene blue-stained samples was excited at 630 nm and registered between 650 and 750 nm. After imaging, the tissue was processed for standard H&E histopathology. The resulting histological and optical images were compared to each other. Results and Conclusions Our findings demonstrate that both tetracycline and methylene blue are suitable for imaging dysplastic and benign nevi. Melanoma is better delineated in the samples stained in methylene blue. Accurate and rapid delineation of melanoma in standard fresh surgical excisions appears feasible. Lasers Surg. Med. 41:10–16, 2009. © 2008 Wiley-Liss, Inc. en_US
dc.language.iso en en_US
dc.title Delineating melanoma using multimodal polarized light imaging en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 201100706 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Lasers in Surgery and Medicine en_US
dc.journal.volume 41 en_US
dc.journal.issue 1 en_US
dc.article.pages 10-16 en_US
dc.identifier.doi http://dx.doi.org/10.1002/lsm.20736 en_US
dc.identifier.ctation Tannous, Z., Al‐Arashi, M., Shah, S., & Yaroslavsky, A. N. (2009). Delineating melanoma using multimodal polarized light imaging. Lasers in surgery and medicine, 41(1), 10-16. en_US
dc.author.email zeina.tannous@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1002/lsm.20736/full en_US
dc.author.affiliation Lebanese American University en_US

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