The role of RhoG in migration and invasion of glioblastoma cells. (c2017)

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dc.contributor.author Ezzeddine, Amani M.
dc.date.accessioned 2017-11-08T11:17:58Z
dc.date.available 2017-11-08T11:17:58Z
dc.date.copyright 2017 en_US
dc.date.issued 2017-11-08
dc.date.submitted 2017-04-28
dc.identifier.uri http://hdl.handle.net/10725/6559
dc.description.abstract Metastasis is the movement of a tumor from its original site to a secondary site. The ability of cancer cells to metastasize depends on their motility and actin-rich membrane protrusions. These protrusions and motility are regulated by Rho GTPases. Rho GTPases are 20KD proteins that act as molecular switches. RhoG, our protein of interest, belongs to this family and is known to almost have the same sequence and function as Rac1. Literature states that RhoG is involved in cell motility, actin cytoskeleton, and many signaling mechanisms. Previous studies showed that RhoG plays a role in the invasive behavior of glioblastoma cells. Also, RhoG was found to be overexpressed in many tumor tissues. In this paper, we studied the role of RhoG in migration and invasion of glioblastoma cells. To study the effect of RhoG on motility, cells were transfected either with si-Luciferase or with si-RhoG (oligo 5 and 8). The results show that upon RhoG knockdown, the motility of Snb-19 cells decreased. This characteristic was further elucidated through immunostaining. Here, the cells were knocked down using si-RhoG and stimulated with PMA. Our results showed that the ruffles are RhoG independent and that the inhibition of RhoG forms stress fibers. Invasion assay also showed a decrease in cell invasion when RhoG was knocked down. Similarly, the adhesion assay revealed a decrease in adhesion of Snb-19 cells upon RhoG knockdown. Finally, a pull-down assay was done for the Rho family of GTPases which showed that RhoG downregulates RhoA and Rac1 activation levels. Indeed, RhoG like other previously tested Rho GTPases is involved in cancer metastasis. It does so by positively regulating cell motility and invasion. Further studies need to be done to elucidate the exact function of RhoG on such characteristics. en_US
dc.language.iso en en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.subject Dissertations, Academic en_US
dc.subject Metastasis en_US
dc.subject Rho GTPases en_US
dc.subject Glioblastoma multiforme en_US
dc.title The role of RhoG in migration and invasion of glioblastoma cells. (c2017) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201004399 en_US
dc.author.commembers Tokajian, Sima
dc.author.commembers Abi Habib, Ralph
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xii, 50 leaves; col. ill.; 30 cm. available at RNL. en_US
dc.author.advisor El Sibai, Mirvat
dc.keywords Metastasis en_US
dc.keywords Rho GTPases en_US
dc.keywords Cell Motility en_US
dc.keywords Invasion en_US
dc.keywords Adhesion en_US
dc.description.bibliographiccitations Bibliography : leaves 40-50. en_US
dc.identifier.doi https://doi.org/10.26756/th.2017.24 en_US
dc.author.email amani.ezzeddine@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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