In vivo real-time confocal reflectance microscopy

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dc.contributor.author Tannous, Zeina
dc.contributor.author Torres, Abel
dc.contributor.author Gonzalez, Salvador
dc.date.accessioned 2017-11-08T09:15:23Z
dc.date.available 2017-11-08T09:15:23Z
dc.date.copyright 2003 en_US
dc.date.issued 2017-11-08
dc.identifier.issn 1524-4725 en_US
dc.identifier.uri http://hdl.handle.net/10725/6542
dc.description.abstract Background Mohs micrographic surgery (MMS) is based on microscopically controlled excision of cutaneous neoplasms and offers the highest cure rates with maximum tissue preservation. In vivo confocal microscopy (CM) allows noninvasive optical imaging of thin sections of living skin, in its native state, in real time, with high resolution and contrast. Objective To evaluate the feasibility of the use of in vivo CM as a surgical guide in MMS. Methods Five patients with a biopsy-proven basal cell carcinoma (BCC) were imaged by in vivo CM on one or two sites from the clinically visible skin cancer. The first Mohs layer was then excised, and the fresh-frozen sections were correlated with the CM findings. Aluminum chloride (AlCl) 20% was applied on the Mohs defect followed by in vivo CM on one site from each lesion. A second Mohs layer was subsequently excised, and fresh-frozen sections were correlated with CM findings. Results The findings of in vivo CM were confirmed by hematoxylin and eosin–stained frozen sections after excisions of the first and second Mohs layers. AlCl was found to provide an excellent contrast between BCC cells and the surrounding tissue, detected readily with both in vivo and ex vivo CM. The tumor cells with AlCl exhibited intensely bright nuclei with an excellent contrast as compared with the low-contrast dark nuclei without AlCl application. Conclusion In vivo CM is a potential surgical guide for MMS, and AlCl provides an excellent exogenous agent to enhance tumor contrast for CM. en_US
dc.language.iso en en_US
dc.title In vivo real-time confocal reflectance microscopy en_US
dc.type Article en_US
dc.description.version Published en_US
dc.title.subtitle A noninvasive guide for mohs micrographic surgery facilitated by aluminum chloride, an excellent contrast enhancer en_US
dc.author.school SOM en_US
dc.author.idnumber 201100706 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Dermatologic Surgery en_US
dc.journal.volume 29 en_US
dc.journal.issue 8 en_US
dc.article.pages 839-846 en_US
dc.identifier.doi http://dx.doi.org/10.1046/j.1524-4725.2003.29219.x en_US
dc.identifier.ctation Tannous, Z., Torres, A., & González, S. (2003). In Vivo Real‐Time Confocal Reflectance Microscopy: A Noninvasive Guide for Mohs Micrographic Surgery Facilitated by Aluminum Chloride, an Excellent Contrast Enhancer. Dermatologic surgery, 29(8), 839-846. en_US
dc.author.email zeina.tannous@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1046/j.1524-4725.2003.29219.x/full en_US
dc.author.affiliation Lebanese American University en_US

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