Characterization of HWP2 a putative GPI anchored candida albicans cell surface protein. (c2007)

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dc.contributor.author Hayek, Peter Jose
dc.date.accessioned 2011-09-27T07:37:18Z
dc.date.available 2011-09-27T07:37:18Z
dc.date.copyright 2007 en_US
dc.date.issued 2011-09-27
dc.date.submitted 2007-09-24
dc.identifier.uri http://hdl.handle.net/10725/637
dc.description Includes bibliographical references (leaves 46-54). en_US
dc.description.abstract The yeast Candida albicans is one of the leading fungal pathogens caus1l1g death in immunocompromised individuals. Various factors are thought to be responsible for vi rulence, such as lipases, proteases and adhesins. Many of these factors are glycosylphosphatidylinositol (GPI) anchored cell surface antigenic determinant proteins responsible for pathogenicity. Hwp2 is a putative GPI anchored protein. The purpose of this study is to characterize the role of Hwp2 as far as filamentation on solid and liquid media, virulence in a mouse model of disseminated candidiasis, drug resistance to six widely used antifungal agents, and white opaque switching, by creating a homozygous null hwp2 strain. It was observed that an hwp2LJ strain was highly filamentation deficient on corn meal and sabouraud solid agar media but not on most liquid media tested, with 100% serum being a minor exception. Furthermore the mutant strain was slightly reduced in virulence compared to the wild strain since all mice infected with the control strain died after 6 days of injection compared with 11 days for the mutant. No discrepancy between the control and mutant was observed as far as drug resistance using the E-test method, or white opaque switching. These results indicate a possible role for Hwp2 in adhesion and invasiveness. Finally aligning the Hwp2 sequence with its two closest homologues, Hwp 1 and Rbtl revealed a small conserved region of high homology found twice in each protein that might reflect a possible sequence necessary for structural integrity. en_US
dc.language.iso en en_US
dc.subject Candida albicans en_US
dc.subject Microbial genetics en_US
dc.subject Antifungal agents en_US
dc.subject Drug Resistance, Fungal en_US
dc.title Characterization of HWP2 a putative GPI anchored candida albicans cell surface protein. (c2007) en_US
dc.type Thesis en_US
dc.term.submitted Summer II en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school Arts and Sciences en_US
dc.author.idnumber 200402695 en_US
dc.author.commembers Dr. Costantine Daher
dc.author.commembers Dr. Sima Tokajian
dc.author.woa OA en_US
dc.description.physdesc 1 bound copy: vii, 54 leaves; col. ill.; 30 cm. available at RNL. en_US
dc.author.division Biology en_US
dc.author.advisor Dr. Roy Khalaf
dc.identifier.doi https://doi.org/10.26756/th.2007.17 en_US
dc.publisher.institution Lebanese American University en_US

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