Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes

LAUR Repository

Show simple item record

dc.contributor.author Leroy, Jerome
dc.contributor.author Abi-Gerges, Aniella
dc.contributor.author Richter, Wito
dc.contributor.author Nikolaev, Viacheslav O.
dc.contributor.author Engelhardt, Stefan
dc.contributor.author Lohse, Martin J.
dc.contributor.author Conti, Marco
dc.contributor.author Fischmeister, Rodolphe
dc.contributor.author Vandecasteele, Gregoire
dc.date.accessioned 2017-10-19T12:43:47Z
dc.date.available 2017-10-19T12:43:47Z
dc.date.copyright 2007 en_US
dc.date.issued 2017-10-19
dc.identifier.issn 0022-2828 en_US
dc.identifier.uri http://hdl.handle.net/10725/6362
dc.description.abstract β-Adrenergic stimulation increases cAMP in cardiac cells to induce PKA-mediated phosphorylation of various proteins regulating heart function, such as the L-type Ca 2+ channels which carry I Ca,L and trigger cardiac contraction. Phosphodiesterases (PDEs) 3 and 4 are the main cAMP degrading enzymes in cardiomyocytes and modulate the β-adrenergic regulation of I Ca,L but the significance of this dual regulation remains elusive. Here, we compared the participation of PDE3 and PDE4 in the β-adrenergic regulation of intracellular cAMP and I Ca,L using the FRET-based indicator Epac2-cAMPS and the whole-cell patch-clamp technique in adult rat ventricular myocytes. Isoprenaline (Iso, 100 nM, 15 s) induced a transient increase in both [cAMP] i and I Ca,L. PDE3 inhibition with cilostamide (1 μM) neither modified [cAMP] i nor I Ca,L transients, whereas PDE4 inhibition with Ro-201724 (10 μM) prolonged both signals. When both PDE3 and PDE4 were blocked, [cAMP] i and I Ca,L signals became nearly stable. PKA inhibition with PKI prolonged the β-adrenergic cAMP transient and this effect was not additive with that of Ro201724. Thus, PDE4 is predominant to terminate β-adrenergic induced [cAMP] i increase and I Ca,L stimulation in adult rat cardiomyocytes. en_US
dc.language.iso en en_US
dc.title Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa,L in adult rat ventricular myocytes en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 201402416 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Molecular and Cellular Cardiology en_US
dc.journal.volume 42 en_US
dc.journal.issue 6, Suppl. en_US
dc.article.pages S49 en_US
dc.identifier.doi http://dx.doi.org/10.1117/j.yjmcc.2007.03.139 en_US
dc.identifier.ctation Leroy, J., Abi-Gerges, A., Richter, W., Nikolaev, V. O., Engelhardt, S., Lohse, M. J., ... & Vandecasteele, G. (2007). Role of PDE3 and PDE4 for β-adrenergic control of cAMP and ICa, L in adult rat ventricular myocytes. Journal of Molecular and Cellular Cardiology, 42(6), S49. en_US
dc.author.email aniella.abigerges@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://www.jmmc-online.com/article/S0022-2828(07)00195-2/abstract#articleInformation en_US
dc.orcid.id https://orcid.org/0000-0001-9974-4023 en_US
dc.author.affiliation Lebanese American University en_US

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search LAUR

Advanced Search


My Account