Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA

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dc.contributor.author Capomacchia, Anthony C.
dc.contributor.author Schulman, Stephen G.
dc.date.accessioned 2017-10-18T08:46:40Z
dc.date.available 2017-10-18T08:46:40Z
dc.date.copyright 1978 en_US
dc.date.issued 2017-10-18
dc.identifier.issn 1520-6017 en_US
dc.identifier.uri http://hdl.handle.net/10725/6347
dc.description.abstract The complexations of the singly charged cations of 3-aminoacridine and 7-aminoquinoline by double-stranded, calf thymus DNA were studied by electronic absorption spectrophotometry. At high ratios of total DNA phosphate concentration to total probe concentration (the region associated with intercalative binding), four DNA phosphate units are associated with each bound monocation. However, a binding isotherm based on four DNA phosphate groups in a single binding site does not yield a reproducible equilibrium constant for the binding process. Rather, the sequestration of the monocations by two binding sites, each containing two DNA phosphate units, yields a satisfactory equilibrium expression. This result suggests that the intercalative mode of binding by DNA is not a simple one-step process, which is in agreement with previous kinetic studies. In the region of low DNA phosphate concentration to monocation concentration (the external binding region), the binding is described adequately by a linear isotherm. en_US
dc.language.iso en en_US
dc.title Binding of 3-aminoacridinium and 7-aminoquinolinium monocations to double-stranded DNA en_US
dc.type Article en_US
dc.description.version Published en_US
dc.title.subtitle evidence for nonlinear binding isotherm in intercalative region en_US
dc.author.school SOP en_US
dc.author.idnumber 201408778 en_US
dc.author.department Pharmaceutical Sciences Department en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Pharmaceutical Sciences en_US
dc.journal.volume 67 en_US
dc.journal.issue 1 en_US
dc.article.pages 89-94 en_US
dc.identifier.doi http://dx.doi.org/10.1002/jps.2600670123 en_US
dc.identifier.ctation Capomacchia, A. C., & Schulman, S. G. (1978). Binding of 3‐aminoacridinium and 7‐aminoquinolinium monocations to double‐stranded DNA: Evidence for nonlinear binding isotherm in intercalative region. Journal of pharmaceutical sciences, 67(1), 89-94. en_US
dc.author.email anthony.capomacchia@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1002/jps.2600670123/full en_US
dc.author.affiliation Lebanese American University en_US

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