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Use of stop-flow oxalate ester chemiluminescence as a means to determine conditions for high-performance liquid chromatography chemiluminescence detection of retinoids using normal-phase chromatography

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dc.contributor.author Capomacchia, Anthony C.
dc.contributor.author Bryant, Peter D.
dc.date.accessioned 2017-10-18T06:19:26Z
dc.date.available 2017-10-18T06:19:26Z
dc.date.copyright 1991 en_US
dc.identifier.issn 1873-264X en_US
dc.identifier.uri http://hdl.handle.net/10725/6339
dc.description.abstract Stop-flow chemiluminescence (CL) has been used to determine the conditions necessary for the oxalate ester CL detection of selected retinoids after separation by normal-phase HPLC. Also, the detection of the selected retinoids by bis(2,4,6-trichlorophenyl) oxalate (TCPO)-hydrogen peroxide (H2O2) chemiluminescence and fluorescence (FL) are compared. Stop-flow CL was performed on a prototype unit from High-Tech Scientific, Ltd (Salisbury, UK). Detection limits were determined for retinyl palmitate, retinyl acetate, retinol, etretinate, acitretin and tretinoin, after separation on a YMC PVA-sil, 25 × cm 4.6 mm column using hexane-tetrahydrofuran-acetic acid (75:25:0.01, v/v/v) as eluent at 1.5 ml min−1. A Schoeffel 970 detector was used for both CL and FL detection. Detection by FL was determined with γex 355 nm and λem > 418 nm. CL detection was performed with the deuterium lamp off and no emission filter. CL was induced by mixing the reagents post-column. Pump A — hexane-THF-AcOH (75:25:0.01, v/v/v) at 1.5 ml min−1 pump B — 70 mM TCPO in THF at 0.5 ml min−1 pump C — THF-50% aq H2O2 (75:25, v/v) 1.0 mg ml−1 imidazole at 1.0 ml−1 imidazole at 1.0 ml min−1. en_US
dc.language.iso en en_US
dc.title Use of stop-flow oxalate ester chemiluminescence as a means to determine conditions for high-performance liquid chromatography chemiluminescence detection of retinoids using normal-phase chromatography en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOP en_US
dc.author.idnumber 201408778 en_US
dc.author.department Pharmacy Practice Department en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Pharmaceutical and Biomedical Analysis en_US
dc.journal.volume 9 en_US
dc.journal.issue 10-12 en_US
dc.article.pages 855-860 en_US
dc.keywords Chemiluminescence en_US
dc.keywords Fluorescence en_US
dc.keywords Oxalate ester en_US
dc.keywords Retinoids en_US
dc.keywords Stop-flow chemiluminescence en_US
dc.keywords HPLC-CL detection en_US
dc.identifier.doi https://doi.org/10.1016/0731-7085(91)80013-Y en_US
dc.identifier.ctation Bryant, P. D., & Capomacchia, A. C. (1991). Use of stop-flow oxalate ester chemiluminescence as a means to determine conditions for high-performance liquid chromatography chemiluminescence detection of retinoids using normal-phase chromatography. Journal of pharmaceutical and biomedical analysis, 9(10), 855-860. en_US
dc.author.email anthony.capomacchia@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://www.sciencedirect.com/science/article/pii/073170859180013Y en_US
dc.author.affiliation Lebanese American University en_US


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