A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds

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dc.contributor.author Khalil, C.
dc.contributor.author Hayes, A.
dc.contributor.author Bakand, S.
dc.contributor.author Winder, C.
dc.date.accessioned 2017-10-17T09:25:27Z
dc.date.available 2017-10-17T09:25:27Z
dc.date.copyright 2006 en_US
dc.identifier.issn 1464-0333 en_US
dc.identifier.uri http://hdl.handle.net/10725/6325
dc.description.abstract Exposure to vapours of volatile chemicals is a major occupational and environmental health concern. Toxicity testing of volatile organic compounds (VOCs) has always faced significant technological problems due to their high volatility and/or low solubility. The aim of this study was to develop a practical and reproducible in vitro exposure technique for toxicity testing of VOCs. Standard test atmospheres of xylene and toluene were generated in glass chambers using a static method. Human cells including: A549-lung derived cell lines, HepG2-liver derived cell lines and skin fibroblasts, were grown in porous membranes and exposed to various airborne concentrations of selected VOCs directly at the air/liquid interface for 1 h at 37 °C. Cytotoxicity of test chemicals was investigated using the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) and NRU (neutral red uptake) assays following 24 h incubation. Airborne IC50 (50% inhibitory concentration) values were determined using dose response curves for xylene (IC50 = 5350 ± 328 ppm, NRU; IC50 = 5750 ± 433 ppm, MTS in skin fibroblast) and toluene (IC50 = 10[thin space (1/6-em)]500 ± 527 ppm, NRU; IC50 = 11[thin space (1/6-em)]200 ± 1044 ppm, MTS in skin fibroblast). Our findings suggest that static direct exposure at the air/liquid interface is a practical and reproducible technique for toxicity testing of VOCs. Further, this technique can be used for inhalational and dermal toxicity studies of volatile chemicals in vitro as the exposure pattern in vivo is closely simulated by this method. en_US
dc.language.iso en en_US
dc.title A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201408580 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Environmental Monitoring en_US
dc.journal.volume 8 en_US
dc.journal.issue 1 en_US
dc.article.pages 100-105 en_US
dc.identifier.ctation Bakand, S., Winder, C., Khalil, C., & Hayes, A. (2006). A novel in vitro exposure technique for toxicity testing of selected volatile organic compounds. Journal of Environmental Monitoring, 8(1), 100-105. en_US
dc.author.email christian.khalil@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://pubs.rsc.org/-/content/articlehtml/2006/em/b509812b en_US
dc.author.affiliation Lebanese American University en_US

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