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In vitro cytotoxicity of formaldehyde and glutaraldehyde mixtures in human cells

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dc.contributor.author Khalil, Christian
dc.contributor.author Bakand, Shahnaz
dc.contributor.author Winder, Chris
dc.contributor.author Hayes, Amanda
dc.date.accessioned 2017-10-17T09:17:28Z
dc.date.available 2017-10-17T09:17:28Z
dc.date.copyright 2006 en_US
dc.identifier.uri http://hdl.handle.net/10725/6324
dc.description.abstract The cytotoxicity of formaldehyde, glutaraldehyde and their binary mixtures was determined using the MTS (tetrazolium salt; Promega) in vitro assay. Cytotoxicity endpoints were investigated in human cells including; pulmonary type II-like epithelial cell lines (A549), hepatoma cell lines (HepG2) and skin fibroblasts. In order to study the cytotoxic effects of airborne formaldehyde, standard atmospheres at concentrations below 10 ppm (12.3 mg/m3 ) were generated using a dynamic diffusion method. Formaldehyde was bubbled through serum free culture media and cell viability was investigated after treating cells with formaldehyde air samples. HepG2 cells were found to be more sensitive (IC50 = 103.8 ± 23.6 mg/L) to formaldehyde cytotoxicity than both A549 lung derived cells and skin fibroblasts (P < 0.01). For glutaraldehyde, skin fibroblasts were found to be more sensitive (IC50 = 99.9 ± 17.2 mg/L) than A549 cells (p < 0.01). Mixtures of formaldehyde and glutaraldehyde at a specific range of concentrations, approximately 5-20% (w/w) formaldehyde, induced synergistic effects in A549 cells. Exposing of HepG2 cells with formaldehyde air samples (8.75 ppm × 4 h) reduced cell viability to less than 50% (31.6 ± 1.24%). Our findings emphasized the toxic effects of formaldehyde and glutaraldehyde and their potential toxicity interactions should be taken into consideration for occupational risk assessment of health care workers. en_US
dc.language.iso en en_US
dc.title In vitro cytotoxicity of formaldehyde and glutaraldehyde mixtures in human cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201408580 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of UOEH en_US
dc.journal.volume 28 en_US
dc.journal.issue Supplement May 20 en_US
dc.article.pages 173-178 en_US
dc.keywords Cytotoxicity en_US
dc.keywords Formaldehyde en_US
dc.keywords Vitro en_US
dc.keywords Glutaraldehyde en_US
dc.keywords Cells en_US
dc.keywords Mixtures en_US
dc.keywords Human en_US
dc.identifier.ctation Bakand, S., Winder, C., Khalil, C., & Hayes, A. (2006). In vitro cytotoxicity of Formaldehyde and Glutaraldehyde mixtures in human cells. en_US
dc.author.email christian.khalil@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://ro.uow.edu.au/sspapers/3155/ en_US
dc.author.affiliation Lebanese American University en_US


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