The ras/cAMP-dependent protein kinase signaling pathway regulates an early step of the autophagy process in saccharomyces

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dc.contributor.author Stephan, Joseph S.
dc.contributor.author Budovskyaya, Yelena V.
dc.contributor.author Reggiori, Fulvio
dc.contributor.author Klionsky, Daniel J.
dc.contributor.author Herman, Paul K.
dc.date.accessioned 2017-09-22T08:38:53Z
dc.date.available 2017-09-22T08:38:53Z
dc.date.copyright 2004 en_US
dc.date.issued 2017-09-22
dc.identifier.issn 1083-351X en_US
dc.identifier.uri http://hdl.handle.net/10725/6235
dc.description.abstract When faced with nutrient deprivation, Saccharomyces cerevisiae cells enter into a nondividing resting state, known as stationary phase. The Ras/PKA (cAMP-dependent protein kinase) signaling pathway plays an important role in regulating the entry into this resting state and the subsequent survival of stationary phase cells. The survival of these resting cells is also dependent upon autophagy, a membrane trafficking pathway that is induced upon nutrient deprivation. Autophagy is responsible for targeting bulk protein and other cytoplasmic constituents to the vacuolar compartment for their ultimate degradation. The data presented here demonstrate that the Ras/PKA signaling pathway inhibits an early step in autophagy because mutants with elevated levels of Ras/PKA activity fail to accumulate transport intermediates normally associated with this process. Quantitative assays indicate that these increased levels of Ras/PKA signaling activity result in an essentially complete block to autophagy. Interestingly, Ras/PKA activity also inhibited a related process, the cytoplasm to vacuole targeting (Cvt) pathway that is responsible for the delivery of a subset of vacuolar proteins in growing cells. These data therefore indicate that the Ras/PKA signaling pathway is not regulating a switch between the autophagy and Cvt modes of transport. Instead, it is more likely that this signaling pathway is controlling an activity that is required during the early stages of both of these membrane trafficking pathways. Finally, the data suggest that at least a portion of the Ras/PKA effects on stationary phase survival are the result of the regulation of autophagy activity by this signaling pathway en_US
dc.language.iso en en_US
dc.title The ras/cAMP-dependent protein kinase signaling pathway regulates an early step of the autophagy process in saccharomyces en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 201509224 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Biological Chemistry en_US
dc.journal.volume 279 en_US
dc.article.pages 20663-20671 en_US
dc.identifier.doi http://dx.doi.org/10.1074/jbc.M400272200 en_US
dc.identifier.ctation Budovskaya, Y. V., Stephan, J. S., Reggiori, F., Klionsky, D. J., & Herman, P. K. (2004). The Ras/cAMP-dependent protein kinase signaling pathway regulates an early step of the autophagy process in Saccharomyces cerevisiae. Journal of Biological Chemistry, 279(20), 20663-20671. en_US
dc.author.email joseph.stephan@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://www.jbc.org/content/279/20/20663.short en_US
dc.author.affiliation Lebanese American University en_US

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