DNA methylation at selected CpG sites in peripheral blood leukocytes is predictive Of gastric cancer

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dc.contributor.author El Maarri, Osman
dc.contributor.author Dauksa, Albertas
dc.contributor.author Gulbinas, Antanas
dc.contributor.author Enzinas, Zilvinas
dc.contributor.author Oldenburg, Johannes
dc.date.accessioned 2017-09-15T06:44:17Z
dc.date.available 2017-09-15T06:44:17Z
dc.date.copyright 2014 en_US
dc.date.issued 2017-09-15
dc.identifier.issn 1791-7530 en_US
dc.identifier.uri http://hdl.handle.net/10725/6197
dc.description.abstract Background/Aim: Recently, a set of studies addressed the question of the prevalence of aberrant methylation in surrogate tissues, such as peripheral blood leukocytes. Toward this aim, we conducted a case-control pilot study to investigate aberrant methylation in leukocytes of gastric cancer patients. Materials and Methods: The SNuPE combined with ion pair reverse phase HPLC (SIRPH method) was used to examine site-specific methylation status at selected CpG sites of the promoter regions of APC, ACIN1, BCL2, CD44, DAPK1, CDKN2A, RARB, TNFRSF10C HS3ST2 and of LINE-1, Alu repeats. Results: We observed that in the patients, tumor suppressor genes were slightly but significantly higher methylated at several CpG sites, while DNA repetitive elements were slightly less methylated compared to controls. This was found to be significantly associated with higher prevalence for gastric cancer. Conclusion: These results suggest that larger studies must be carried-out to explore the biological significance and clinical usefulness of leukocyte DNA as non-invasive detection tool for gastric cancer. en_US
dc.language.iso en en_US
dc.title DNA methylation at selected CpG sites in peripheral blood leukocytes is predictive Of gastric cancer en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Anticancer Research en_US
dc.journal.volume 34 en_US
dc.journal.issue 10 en_US
dc.article.pages 5381-5388 en_US
dc.keywords DNA methylation en_US
dc.keywords Gastric cancer en_US
dc.keywords Surrogate tissue en_US
dc.keywords Peripheral blood leukocyte en_US
dc.keywords SIRPH en_US
dc.identifier.ctation Dauksa, A., Gulbinas, A., Endzinas, Z., Oldenburg, J., & El-Maarri, O. (2014). DNA methylation at selected CpG sites in peripheral blood leukocytes is predictive of gastric cancer. Anticancer research, 34(10), 5381-5388. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://ar.iiarjournals.org/content/34/10/5381.full.pdf+html en_US
dc.author.affiliation Lebanese American University en_US

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