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Measurements of DNA methylation at seven loci in various tissues of CD1 mice

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dc.contributor.author El Maarri, Osman
dc.contributor.author Daugela, Laurynas
dc.contributor.author Nusgen, Nicole
dc.contributor.author Walier, Maja
dc.contributor.author Oldenburg, Johannes
dc.date.accessioned 2017-09-14T12:22:17Z
dc.date.available 2017-09-14T12:22:17Z
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/10725/6193
dc.description.abstract In humans, considerable variation in methylation at single loci and repetitive elements in various cells and tissues is observed. Recently, several inter- and intra-tissue correlations for DNA methylation have been reported. To investigate the extent and reproducibility of such correlations, we investigated inter- and intra-tissue methylation correlations among seven different loci in 9 different tissues in a population of 100 healthy seven-week-old CD1 outbred mice. We used a highly quantitative approach to measure methylation levels to high accuracy at two single loci in the alpha-actin and myosine light chain promoters, at three differentially methylated regions of the Peg3, Snrpn and Lit1 genes associated with imprinted loci, and at two repetitive elements in the Line-1 and IAP-LTR genes in the various tissues. In this population of mice, methylation at several loci was sex-associated and intra-tissue correlations among the studied loci were observed for brain and spleen. Inter-tissue correlations were rarely observed. To investigate method-dependent experimental variability, we re-analyzed the same spleen and tongue samples using SIRPH and pyrosequencing methods and reconfirmed intra-tissue correlations for spleen and sex-associated correlations for DNA methylation for tongue. When we repeated DNA methylation measurements for a second mouse population raised under similar conditions three months later, we did not detect sex-associated or intra-tissues correlations. Additional studies that examine large numbers of loci may be required to further understand the factors that influence stability of DNA methylation. en_US
dc.language.iso en en_US
dc.title Measurements of DNA methylation at seven loci in various tissues of CD1 mice en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Plos one en_US
dc.journal.volume 7 en_US
dc.journal.issue 9 en_US
dc.article.pages e44585 en_US
dc.identifier.doi https://doi.org/10.1371/journal.pone.0044585 en_US
dc.identifier.ctation Daugela, L., Nüsgen, N., Walier, M., Oldenburg, J., Schwaab, R., & El-Maarri, O. (2012). Measurements of DNA methylation at seven loci in various tissues of CD1 mice. PloS one, 7(9), e44585. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0044585 en_US
dc.author.affiliation Lebanese American University en_US


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