Measurements of DNA methylation at seven loci in various tissues of CD1 mice

LAUR Repository

Show simple item record

dc.contributor.author El Maarri, Osman
dc.contributor.author Daugela, Laurynas
dc.contributor.author Nusgen, Nicole
dc.contributor.author Walier, Maja
dc.contributor.author Oldenburg, Johannes
dc.date.accessioned 2017-09-14T12:22:17Z
dc.date.available 2017-09-14T12:22:17Z
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/10725/6193
dc.description.abstract In humans, considerable variation in methylation at single loci and repetitive elements in various cells and tissues is observed. Recently, several inter- and intra-tissue correlations for DNA methylation have been reported. To investigate the extent and reproducibility of such correlations, we investigated inter- and intra-tissue methylation correlations among seven different loci in 9 different tissues in a population of 100 healthy seven-week-old CD1 outbred mice. We used a highly quantitative approach to measure methylation levels to high accuracy at two single loci in the alpha-actin and myosine light chain promoters, at three differentially methylated regions of the Peg3, Snrpn and Lit1 genes associated with imprinted loci, and at two repetitive elements in the Line-1 and IAP-LTR genes in the various tissues. In this population of mice, methylation at several loci was sex-associated and intra-tissue correlations among the studied loci were observed for brain and spleen. Inter-tissue correlations were rarely observed. To investigate method-dependent experimental variability, we re-analyzed the same spleen and tongue samples using SIRPH and pyrosequencing methods and reconfirmed intra-tissue correlations for spleen and sex-associated correlations for DNA methylation for tongue. When we repeated DNA methylation measurements for a second mouse population raised under similar conditions three months later, we did not detect sex-associated or intra-tissues correlations. Additional studies that examine large numbers of loci may be required to further understand the factors that influence stability of DNA methylation. en_US
dc.language.iso en en_US
dc.title Measurements of DNA methylation at seven loci in various tissues of CD1 mice en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Plos one en_US
dc.journal.volume 7 en_US
dc.journal.issue 9 en_US
dc.article.pages e44585 en_US
dc.identifier.doi https://doi.org/10.1371/journal.pone.0044585 en_US
dc.identifier.ctation Daugela, L., Nüsgen, N., Walier, M., Oldenburg, J., Schwaab, R., & El-Maarri, O. (2012). Measurements of DNA methylation at seven loci in various tissues of CD1 mice. PloS one, 7(9), e44585. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0044585 en_US
dc.author.affiliation Lebanese American University en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search LAUR

Advanced Search


My Account