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Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography

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dc.contributor.author El Maarri, Osman
dc.contributor.author Muller, Jens
dc.contributor.author Oldenburg, Johannes
dc.contributor.author Potzsch, Bernd
dc.date.accessioned 2017-09-13T11:16:09Z
dc.date.available 2017-09-13T11:16:09Z
dc.date.copyright 2008 en_US
dc.identifier.issn 1618-2650 en_US
dc.identifier.uri http://hdl.handle.net/10725/6181
dc.description.abstract Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences. en_US
dc.language.iso en en_US
dc.title Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Analytical and Bioanalytical Chemistry en_US
dc.journal.volume 390 en_US
dc.journal.issue 4 en_US
dc.article.pages 1033-1037 en_US
dc.keywords Aptamer en_US
dc.keywords SELEX en_US
dc.keywords dHPLC WAVE en_US
dc.identifier.doi http://dx.doi.org/10.1007/s00216-007-1699-8 en_US
dc.identifier.ctation Müller, J., El-Maarri, O., Oldenburg, J., Pötzsch, B., & Mayer, G. (2008). Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography. Analytical and bioanalytical chemistry, 390(4), 1033-1037. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://link.springer.com/article/10.1007/s00216-007-1699-8 en_US
dc.author.affiliation Lebanese American University en_US


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