Gender specific differences in levels of DNA methylation at selected loci from human total blood

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dc.contributor.author El Maarri, Osman
dc.contributor.author Becker, Tim
dc.contributor.author Junen, Judith
dc.contributor.author Manzoor, Syed Saadi
dc.contributor.author Diaz-Lacava, Amalia
dc.date.accessioned 2017-09-13T09:23:19Z
dc.date.available 2017-09-13T09:23:19Z
dc.date.copyright 2007 en_US
dc.date.issued 2017-09-13
dc.identifier.issn 1432-1203 en_US
dc.identifier.uri http://hdl.handle.net/10725/6180
dc.description.abstract Abnormal patterns of DNA methylation are observed in many diseases such as tumors and imprinting disorders. Little is known about inter-individual and gender specific variations. Here, we report on accurate and sensitive quantitative measurements of methylation in DNA from total blood in 96 healthy human males and 96 healthy human females. Global methylation was estimated by studying two repetitive DNA elements, namely Line-1 and Alu repeats, while single loci were investigated for three differentially methylated regions (DMRs) at PEG3, NESP55 and H19 imprinted genes and two additional loci at Xq28 (F8 gene) and at 19q13.4 (locus between PEG3 and ubiquitin specific protease 29). We observed inter-individual correlations in the degree of methylation between Alu and Line-1 repeats. Moreover, all studied CpGs showed slightly higher methylation in males (P < 0.0003–0.0381), with the exception of DMRs at imprinted genes (P = 0.0342–0.9616) which were almost equally methylated in both sexes with only a small tendency towards higher methylation in males. This observed difference could be due to the process of X chromosome inactivation or merely to the presence of an additional X chromosome in female cells or could be a result of downstream effects of sex determination. en_US
dc.language.iso en en_US
dc.title Gender specific differences in levels of DNA methylation at selected loci from human total blood en_US
dc.type Article en_US
dc.description.version Published en_US
dc.title.subtitle a tendency toward higher methylation levels in males en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Human Genetics en_US
dc.journal.volume 122 en_US
dc.journal.issue 5 en_US
dc.article.pages 505-514 en_US
dc.identifier.doi http://dx.doi.org/10.1007/s00439-007-0430-3 en_US
dc.identifier.ctation El-Maarri, O., Becker, T., Junen, J., Manzoor, S. S., Diaz-Lacava, A., Schwaab, R., ... & Oldenburg, J. (2007). Gender specific differences in levels of DNA methylation at selected loci from human total blood: a tendency toward higher methylation levels in males. Human genetics, 122(5), 505-514. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://link.springer.com/article/10.1007/s00439-007-0430-3 en_US
dc.author.affiliation Lebanese American University en_US

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