Epigenetic reprogramming in mouse primordial germ cells

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dc.contributor.author El Maarri, Osman
dc.contributor.author Hajkova, Petra
dc.contributor.author Erhardt, Sylvia
dc.contributor.author Lane, Natasha
dc.contributor.author Haaf, Thomas
dc.contributor.author Reik, Wolf
dc.contributor.author Walter, Jorn
dc.contributor.author Surani, M.Azmi
dc.date.accessioned 2017-09-12T11:40:45Z
dc.date.available 2017-09-12T11:40:45Z
dc.date.copyright 2002 en_US
dc.date.issued 2017-09-12
dc.identifier.issn 1872-6356 en_US
dc.identifier.uri http://hdl.handle.net/10725/6168
dc.description.abstract Genome-wide epigenetic reprogramming in mammalian germ cells, zygote and early embryos, plays a crucial role in regulating genome functions at critical stages of development. We show here that mouse primordial germ cells (PGCs) exhibit dynamic changes in epigenetic modifications between days 10.5 and 12.5 post coitum (dpc). First, contrary to previous suggestions, we show that PGCs do indeed acquire genome-wide de novo methylation during early development and migration into the genital ridge. However, following their entry into the genital ridge, there is rapid erasure of DNA methylation of regions within imprinted and non-imprinted loci. For most genes, the erasure commences simultaneously in PGCs in both male and female embryos, which is completed within 1 day of development. Based on the kinetics of this process, we suggest that this is an active demethylation process initiated upon the entry of PGCs into the gonadal anlagen. The timing of reprogramming in PGCs is crucial since it ensures that germ cells of both sexes acquire an equivalent epigenetic state prior to the differentiation of the definitive male and female germ cells in which new parental imprints are established subsequently. Some repetitive elements, however, show incomplete erasure, which may be essential for chromosome stability and for preventing activation of transposons to reduce the risk of germline mutations. Aberrant epigenetic reprogramming in the germ line would cause the inheritance of epimutations that may have consequences for human diseases as suggested by studies on mouse models. en_US
dc.language.iso en en_US
dc.title Epigenetic reprogramming in mouse primordial germ cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Mechanisms of Development en_US
dc.journal.volume 117 en_US
dc.journal.issue 1-2 en_US
dc.article.pages 15-23 en_US
dc.identifier.doi https://doi.org/10.1016/S0925-4773(02)00181-8 en_US
dc.identifier.ctation Hajkova, P., Erhardt, S., Lane, N., Haaf, T., El-Maarri, O., Reik, W., ... & Surani, M. A. (2002). Epigenetic reprogramming in mouse primordial germ cells. Mechanisms of development, 117(1), 15-23. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S0925477302001818 en_US
dc.author.affiliation Lebanese American University en_US

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