A rapid, quantitative, non-radioactive bisulfite-SNuPE- IP RP HPLC assay for methylation analysis at specific CpG sites

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dc.contributor.author El Maarri, Osman
dc.contributor.author Herbiniaux, Ursula
dc.contributor.author Walter, Jorn
dc.contributor.author Oldenburg, Johannes
dc.date.accessioned 2017-09-12T11:31:51Z
dc.date.available 2017-09-12T11:31:51Z
dc.date.copyright 2002 en_US
dc.date.issued 2017-09-12
dc.identifier.issn 1362-4962 en_US
dc.identifier.uri http://hdl.handle.net/10725/6167
dc.description.abstract The precise mapping and quantification of DNA methylation as an epigenetic parameter during development and in diseased tissues is of great importance for functional genomics. Here we describe a rapid, quantitative method to assess methylation levels at specific CpG sites using PCR products of bisulfite-treated genomic DNA. Using single nucleotide primer extension (SNuPE) assays in combination with ion pair reverse phase high performance liquid chromatography (IP RP HPLC) separation techniques, methylated and unmethylated CpGs can be discriminated and quantified based on the different masses and hydrophobicities of the extended primer products. The assay is linear, highly reproducible and several sites can be measured simultaneously in one reaction. It can be semi-automated and eliminates the need for cloning and sequencing of individual bisulfite PCR products. en_US
dc.language.iso en en_US
dc.title A rapid, quantitative, non-radioactive bisulfite-SNuPE- IP RP HPLC assay for methylation analysis at specific CpG sites en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Nucleic Acids Research en_US
dc.journal.volume 30 en_US
dc.journal.issue 6 en_US
dc.article.pages e25 en_US
dc.identifier.doi https://doi.org/10.1093/nar/30.6.e25 en_US
dc.identifier.ctation El-Maarri, O., Herbiniaux, U., Walter, J., & Oldenburg, J. (2002). A rapid, quantitative, non-radioactive bisulfite-SNuPE-IP RP HPLC assay for methylation analysis at specific CpG sites. Nucleic Acids Research, 30(6), e25-e25. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://doi.org/10.1093/nar/30.6.e25 en_US
dc.author.affiliation Lebanese American University en_US

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