DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos

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dc.contributor.author El Maarri, Osman
dc.contributor.author Stancheva, Irina
dc.contributor.author Walter, Joern
dc.contributor.author Niveleau, Alain
dc.contributor.author Meehan, Richard R.
dc.date.accessioned 2017-09-12T11:09:51Z
dc.date.available 2017-09-12T11:09:51Z
dc.date.copyright 2002 en_US
dc.date.issued 2017-09-12
dc.identifier.issn 1095-564X en_US
dc.identifier.uri http://hdl.handle.net/10725/6166
dc.description.abstract The levels of genomic DNA methylation in vertebrate species display a wide range of developmental dynamics. Here, we show that in contrast to mice, the paternal genome of the amphibian, Xenopus laevis, is not subjected to active demethylation of 5-methyl cytosine immediately after fertilization. High levels of methylation in the DNA of both oocyte and sperm are maintained in the early embryo but progressively decline during the cleavage stages. As a result, the Xenopus genome has its lowest methylation content at the midblastula transition (MBT) and during subsequent gastrulation. Between blastula and gastrula stages, we detect a loss of methylation at individual Xenopus gene promoters (TFIIIA, Xbra, and c-Myc II) that are activated at MBT. No changes are observed in the methylation patterns of repeated sequences, genes that are inactive at MBT, or in the coding regions of individual genes. In embryos that are depleted of the maintenance methyltransferase enzyme (xDnmt1), these developmentally programmed changes in promoter methylation are disrupted, which may account for the altered patterns of gene expression that occur in these embryos. Our results suggest that DNA methylation has a role in regulating the timing of gene activation at MBT in Xenopus laevis embryos. en_US
dc.language.iso en en_US
dc.title DNA methylation at promoter regions regulates the timing of gene activation in xenopus laevis embryos en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Developmental Biology en_US
dc.journal.volume 243 en_US
dc.journal.issue 1 en_US
dc.article.pages 155-165 en_US
dc.keywords Xenopus en_US
dc.keywords Development en_US
dc.keywords Demethylation en_US
dc.keywords xDnmt1 en_US
dc.identifier.doi https://doi.org/10.1006/dbio.2001.0560 en_US
dc.identifier.ctation Stancheva, I., El-Maarri, O., Walter, J., Niveleau, A., & Meehan, R. R. (2002). DNA methylation at promoter regions regulates the timing of gene activation in Xenopus laevis embryos. Developmental biology, 243(1), 155-165. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S0012160601905603 en_US
dc.author.affiliation Lebanese American University en_US

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