Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene

El Maarri, Osman; Oldenburg, Johannes; Caglayan, S.Hande

Intron 22-specific long PCR for the Xba I polymorphism in the factor VIII gene

El Maarri, Osman; Oldenburg, Johannes; Caglayan, S.Hande

URI: http://hdl.handle.net/10725/6162

URL: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2141.1999.01430.x/full

DOI: http://dx.doi.org/10.1046/j.1365-2141.1999.01430.x

Date: 2017-09-12

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Abstract:

The Xba I polymorphic site in the factor VIII gene is present in the int22h-1 region which is found in two other copies (int22h-2 and int22h-3) distal to the gene. Previously the polymorphic status of the Xba I locus was studied by either Southern blot or PCR that amplified all three copies. Here we report the use of a long PCR that specifically amplifies the intragenic site in intron 22, making use of this marker an easy and reliable assay. Moreover, about 25% of previously uninformative Turkish haemophilia A families examined with three markers proved to be informative for linkage analysis, using the Xba I polymorphism.

Citation:

El‐Maarri, O., Oldenburg, J., & Çağlayan, S. H. (1999). Intron 22‐specific long PCR for the Xba I polymorphism in the factor VIII gene. British journal of haematology, 105(4), 1120-1122.