Analysis of the two microsatellite repeat polymorphisms of the factor VIII gene in the Turkish population

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dc.contributor.author El Maarri, Osman
dc.contributor.author Jarjanazi, Hamdi
dc.contributor.author Timur, A.Anil
dc.contributor.author Caglayan, S.Hande
dc.date.accessioned 2017-09-12T06:09:51Z
dc.date.available 2017-09-12T06:09:51Z
dc.date.copyright 1998 en_US
dc.date.issued 2017-09-12
dc.identifier.issn 1365-2141 en_US
dc.identifier.uri http://hdl.handle.net/10725/6160
dc.description.abstract DNA-based diagnosis of haemophilia A has previously been carried out by linkage analysis using two highly informative markers, Hind III RFLP and St14 VNTR, for affected Turkish families. In the present study the number and frequency of the microsatellite alleles at introns 13 and 22 in the factor VIII (FVIII) gene were analysed in order to increase the rate of informative females and accuracy of linkage analysis. Six alleles were observed at both loci. The two most frequent alleles of each locus were the same as the two common alleles found in Anglo-Americans. The comparison of heterozygosity of both microsatellite loci showed that the Turkish population is slightly less polymorphic than Anglo-Americans but more polymorphic than Chinese, Slavs and Uzbekians. The additional use of the two microsatellite repeat polymorphisms with the previously established informative markers has been accepted as the most effective strategy in DNA diagnosis by linkage analysis for the assessment of haemophilia A carriers and affected fetuses in the Turkish population. The modifications adopted in this study for the multiplex PCR analysis of the microsatellite repeat polymorphism eliminated the use of radioactivity and sequencing gels, reducing cost and labour. en_US
dc.language.iso en en_US
dc.title Analysis of the two microsatellite repeat polymorphisms of the factor VIII gene in the Turkish population en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201508713 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal British journal of Haematology en_US
dc.journal.volume 100 en_US
dc.journal.issue 3 en_US
dc.article.pages 589-593 en_US
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-2141.1998.00588.x en_US
dc.identifier.ctation Timur, A. A., & Çağlayan, S. H. (1998). Analysis of the two microsatellite repeat polymorphisms of the factor VIII gene in the Turkish population. British journal of haematology, 100(3), 589-593. en_US
dc.author.email osman.elmaarri@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2141.1998.00588.x/full en_US
dc.author.affiliation Lebanese American University en_US

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