Alteration of the DNA binding domain disrupts distinct functions of the C. elegans Pax protein EGL-38

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dc.contributor.author Sleiman, Sama F.
dc.contributor.author Zhang, Guojuan
dc.contributor.author Tseng, Ronj-Jeng
dc.contributor.author Rajakumar, Vandana
dc.contributor.author Wang, Xiadong
dc.contributor.author Chamberlin, Helen M.
dc.date.accessioned 2017-09-06T09:38:53Z
dc.date.available 2017-09-06T09:38:53Z
dc.date.copyright 2005 en_US
dc.date.issued 2017-09-06
dc.identifier.issn 1872-6356 en_US
dc.identifier.uri http://hdl.handle.net/10725/6145
dc.description.abstract The paired-domain-containing Pax transcription factors play an important role in the development of a range of organ, tissue and cell types. Although DNA binding elements and target genes for Pax proteins have been identified, how these proteins identify appropriate DNA elements and regulate different genes in different cellular contexts is not well understood. To investigate the relationship between Pax proteins and their targets, we have studied the in vivo and in vitro properties associated with wild-type and different mutant variants of the Caenorhabditis elegans Pax protein EGL-38. Here, we characterize the properties of four mutations that result in an amino acid substitution in the DNA binding domain of EGL-38. We find that animals bearing the different mutant alleles exhibit tissue-preferential defects in egl-38 function. The mutant proteins are also altered in their activity in an ectopic expression assay and in their in vitro DNA binding properties. Using in vitro selection, we have identified binding sites for EGL-38. However, we show that selected sites function poorly in vivo as EGL-38 response elements, indicating that sequence features in addition to DNA binding determine the efficacy of Pax response elements. The distinction between DNA binding and activity is consistent with the model that other factors commonly play a role in mediating Pax protein target site selection and function in vivo. en_US
dc.language.iso en en_US
dc.title Alteration of the DNA binding domain disrupts distinct functions of the C. elegans Pax protein EGL-38 en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201408170 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Mechanisms of Development en_US
dc.journal.volume 122 en_US
dc.journal.issue 7-8 en_US
dc.article.pages 887-899 en_US
dc.keywords DNA binding en_US
dc.keywords Transactivation en_US
dc.keywords Transcriptional control en_US
dc.keywords Pax transcription factor en_US
dc.identifier.doi https://doi.org/10.1016/j.mod.2005.04.005 en_US
dc.identifier.ctation Zhang, G., Sleiman, S. F., Tseng, R. J., Rajakumar, V., Wang, X., & Chamberlin, H. M. (2005). Alteration of the DNA binding domain disrupts distinct functions of the C. elegans Pax protein EGL-38. Mechanisms of development, 122(7), 887-899. en_US
dc.author.email sama.sleiman@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S0925477305000572 en_US
dc.author.affiliation Lebanese American University en_US

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