Developmental functions for the Caenorhabditis elegans Sp protein SPTF-3

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dc.contributor.author Sleiman, Sama F.
dc.contributor.author Ulm, Elizabeth A.
dc.contributor.author Chamberlin, Helen M.
dc.date.accessioned 2017-09-06T07:17:11Z
dc.date.available 2017-09-06T07:17:11Z
dc.date.copyright 2011 en_US
dc.date.issued 2017-04-11
dc.identifier.issn 1872-6356 en_US
dc.identifier.uri http://hdl.handle.net/10725/6139
dc.description.abstract Sp factors are important for animal development and the transcriptional regulation of a wide variety of genes. How they influence the developmental decisions of individual cells within the organism, however, is poorly understood. To better understand the developmental functions for Sp transcription factors, we have characterized the functions of Caenorhabditis elegans SPTF-3 using RNAi knockdown and a non-null, hypomorphic mutant allele. We find that disruption of sptf-3 confers a variety of developmental defects, including defects in development of the egg-laying system, oocyte production, and embryonic morphogenesis. sptf-3 mutants exhibit defects in vulval lineage polarity, a phenotype previously only observed in mutants defective in Wnt signaling. We show that the embryonic function of sptf-3 is dependent on germline activity, arguing that the gene has an important maternal contribution to embryonic development. An evaluation of reporter gene expression suggests that SPTF-3 exhibits specificity, in that it can influence the expression of a given gene in some cells but not others, and that SPTF-3 participates in the maintenance of gene expression states in differentiated cells. We propose SPTF-3 provides a good model to study the in vivo functions for Sp transcription factors during animal development. en_US
dc.language.iso en en_US
dc.title Developmental functions for the Caenorhabditis elegans Sp protein SPTF-3 en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 201408170 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Mechanisms of Development en_US
dc.journal.volume 128 en_US
dc.journal.issue 7-10 en_US
dc.article.pages 428-441 en_US
dc.keywords Maintenance of gene transcription en_US
dc.keywords Sp family transcription factor en_US
dc.keywords Cell differentiation en_US
dc.keywords Caenorhabditis elegans en_US
dc.identifier.doi https://doi.org/10.1016/j.mod.2011.08.003 en_US
dc.identifier.ctation Ulm, E. A., Sleiman, S. F., & Chamberlin, H. M. (2011). Developmental functions for the Caenorhabditis elegans Sp protein SPTF-3. Mechanisms of development, 128(7), 428-441. en_US
dc.author.email sama.sleiman@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S0925477311000955 en_US
dc.author.affiliation Lebanese American University en_US

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