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WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells

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dc.contributor.author El-Sibai, Mirvat
dc.contributor.author Sarmiento, Corina
dc.contributor.author Wang, Weigang
dc.contributor.author Dovas, Athanassios
dc.contributor.author Yamaguchi, Hideki
dc.contributor.author Sidani, Mazen
dc.contributor.author Desmarais, Vera
dc.contributor.author Holman, Holly A.
dc.contributor.author Kitchen, Susan
dc.contributor.author Backer, Jonathan M.
dc.contributor.author Alberts, Art
dc.contributor.author Condeelis, John
dc.date.accessioned 2017-02-08T13:02:09Z
dc.date.available 2017-02-08T13:02:09Z
dc.date.copyright 2008 en_US
dc.identifier.issn 0021-9525 en_US
dc.identifier.uri http://hdl.handle.net/10725/5198
dc.description.abstract We examined the role of the actin nucleation promoters neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE2 in cell protrusion in response to epidermal growth factor (EGF), a key regulator in carcinoma cell invasion. We found that WAVE2 knockdown (KD) suppresses lamellipod formation and increases filopod formation, whereas N-WASP KD has no effect. However, simultaneous KD of both proteins results in the formation of large jagged protrusions with lamellar properties and increased filopod formation. This suggests that another actin nucleation activity is at work in carcinoma cells in response to EGF. A mammalian Diaphanous–related formin, mDia1, localizes at the jagged protrusions in double KD cells. Constitutively active mDia1 recapitulated the phenotype, whereas inhibition of mDia1 blocked the formation of these protrusions. Increased RhoA activity, which stimulates mDia1 nucleation, was observed in the N-WASP/WAVE2 KD cells and was shown to be required for the N-WASP/WAVE2 KD phenotype. These data show that coordinate regulation between the WASP family and mDia proteins controls the balance between lamellar and lamellipodial protrusion activity. en_US
dc.language.iso en en_US
dc.title WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 200703859 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Cell Biology en_US
dc.journal.volume 180 en_US
dc.journal.issue 6 en_US
dc.article.pages 1 en_US
dc.identifier.doi http://dx.doi.org/110.1083/jcb.200708123 en_US
dc.identifier.ctation Sarmiento, C., Wang, W., Dovas, A., Yamaguchi, H., Sidani, M., El-Sibai, M., ... & Alberts, A. (2008). WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells. The Journal of cell biology, 180(6), 1245-1260. en_US
dc.author.email mirvat.elsibai@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://jcb.rupress.org/content/180/6/1245/tab-pdf en_US
dc.author.affiliation Lebanese American University en_US


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