The identification and analysis of phosphorylation sites on the Atg1 protein kinase

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dc.contributor.author Stephan, Joseph S.
dc.contributor.author Yeh, Yuh-Ying
dc.contributor.author Shah, Khyati H.
dc.contributor.author Chou, Chi-Chi
dc.contributor.author Hsiao, He-Hsuan
dc.contributor.author Wrasman, Kristie M.
dc.contributor.author Stamatakos, Demetra
dc.contributor.author Khoo, Kay-Hooi
dc.contributor.author Herman, Paul K.
dc.date.accessioned 2017-02-06T11:55:32Z
dc.date.available 2017-02-06T11:55:32Z
dc.date.copyright 2011 en_US
dc.date.issued 2017-02-06
dc.identifier.issn 1554-8627 en_US
dc.identifier.uri http://hdl.handle.net/10725/5186
dc.description.abstract Autophagy is a conserved, degradative process that has been implicated in a number of human diseases and is a potential target for therapeutic intervention. It is therefore important that we develop a thorough understanding of the mechanisms regulating this trafficking pathway. The Atg1 protein kinase is a key element of this control as a number of signaling pathways target this enzyme and its associated protein partners. These studies have established that Atg1 activities are controlled, at least in part, by protein phosphorylation. To further this understanding, we used a combined mass spectrometry and molecular biology approach to identify and characterize additional sites of phosphorylation in the Saccharomyces cerevisiae Atg1. Fifteen candidate sites of phosphorylation were identified, including nine that had not been noted previously. Interestingly, our data suggest that the phosphorylation at one of these sites, Ser-34, is inhibitory for both Atg1 kinase activity and autophagy. This site is located within a glycine-rich loop that is highly conserved in protein kinases. Phosphorylation at this position in several cyclin-dependent kinases has also been shown to result in diminished enzymatic activity. In addition, these studies identified Ser-390 as the site of autophosphorylation responsible for the anomalous migration exhibited by Atg1 on SDS-polyacrylamide gels. Finally, a mutational analysis suggested that a number of the sites identified here are important for full autophagy activity in vivo. In all, these studies identified a number of potential sites of regulation within Atg1 and will serve as a framework for future work with this enzyme. en_US
dc.language.iso en en_US
dc.title The identification and analysis of phosphorylation sites on the Atg1 protein kinase en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 201509224 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Autophagy en_US
dc.journal.volume 7 en_US
dc.journal.issue 7 en_US
dc.article.pages 716-726 en_US
dc.keywords Protein phosphorylation en_US
dc.keywords Mass spectrometry en_US
dc.keywords Autophosphorylation en_US
dc.keywords Atg1 protein kinase en_US
dc.keywords Autophagy en_US
dc.keywords Activation loop en_US
dc.identifier.doi http://dx.doi.org/10.4161/auto.7.7.15155 en_US
dc.identifier.ctation Yeh, Y. Y., Shah, K. H., Chou, C. C., Hsiao, H. H., Wrasman, K. M., Stephan, J. S., ... & Herman, P. K. (2011). The identification and analysis of phosphorylation sites on the Atg1 protein kinase. Autophagy, 7(7), 716-726. en_US
dc.author.email joseph.stephan@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://www.tandfonline.com/doi/abs/10.4161/auto.7.7.15155 en_US
dc.author.affiliation Lebanese American University en_US

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