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A SAGE (Serial analysis of gene expression) view of breast tumor progression

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dc.contributor.author Nasser, Selim
dc.contributor.author Porter, Dale A.
dc.contributor.author Krop, Ian E.
dc.contributor.author Sgroi, Dennis
dc.contributor.author Kaelin, Carolyn M.
dc.contributor.author Marks, Jeffrey R.
dc.contributor.author Riggins, Gregory
dc.contributor.author Polyak, Kornelia
dc.date.accessioned 2017-02-03T10:31:03Z
dc.date.available 2017-02-03T10:31:03Z
dc.date.copyright 2001 en_US
dc.identifier.issn 0008-5472 en_US
dc.identifier.uri http://hdl.handle.net/10725/5168
dc.description.abstract To identify molecular alterations involved in the initiation and progression of breast carcinomas, we analyzed the global gene expression profiles of normal mammary epithelial cells and in situ, invasive, and metastatic breast carcinomas using serial analysis of gene expression (SAGE). We identified sets of genes expressed only or most abundantly in a specific stage of breast tumorigenesis or in a certain subtype of tumors through the pair-wise comparison and by hierarchical clustering analysis of these eight SAGE libraries (two/stage). On the basis of these comparisons, we made the following observations: Normal mammary epithelial cells showed the most distinct and least variable gene expression profiles. Many of the genes highly expressed in normal mammary epithelium and lost in carcinomas encoded secreted proteins, cytokines, and chemokines, implicating abnormal paracrine and autocrine signaling in the initiation of breast tumorigenesis. Very few genes were universally up-regulated in all tumors regardless of their stage and histological grade, indicating a high degree of diversity at the molecular level that likely reflects the clinical heterogeneity characteristic of breast carcinomas. Tumors of different histology type and stage had very distinct gene expression patterns. No genes seemed to be specific for metastatic or for in situ carcinomas. We found that the most dramatic and consistent phenotypic change occurred at the normal-to-in situ carcinoma transition. This observation, combined with the fact that many of the genes involved encode secreted, cell-nonautonomous factors, implies that the normal epithelium-to-in situ carcinoma transition may be the most promising target for cancer prevention and treatment. en_US
dc.language.iso en en_US
dc.title A SAGE (Serial analysis of gene expression) view of breast tumor progression en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOM en_US
dc.author.idnumber 200804624 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Cancer Research en_US
dc.journal.volume 61 en_US
dc.journal.issue 15 en_US
dc.article.pages 5697-5702
dc.identifier.ctation Porter, D. A., Krop, I. E., Nasser, S., Sgroi, D., Kaelin, C. M., Marks, J. R., ... & Polyak, K. (2001). A SAGE (serial analysis of gene expression) view of breast tumor progression. Cancer Research, 61(15), 5697-5702. en_US
dc.author.email selim.nasser@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://cancerres.aacrjournals.org/content/61/15/5697 en_US
dc.author.affiliation Lebanese American University en_US


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