Production and properties of thermostable proteases from bacillus thermoleovorans strain HSR

LAUR Repository

Show simple item record

dc.contributor.author Tokajian, Sima
dc.contributor.author Hashwa, Fuad
dc.contributor.author Sunna, Anwar
dc.date.accessioned 2017-01-30T10:08:33Z
dc.date.available 2017-01-30T10:08:33Z
dc.date.copyright 2000 en_US
dc.date.issued 2017-01-30
dc.identifier.issn 1561-3410 en_US
dc.identifier.uri http://hdl.handle.net/10725/5110
dc.description.abstract The thermophilic bacterium Bacillus thermoleovorans strain HSR secretes thermostable extracellular proteases when cultivated in the presence of tryptone. The proteases which are found to be of the metallo-protease type, showed maximal activity when incubated at 80°C and pH 7.0. The enzymes are thermostable at temperatures between 60 and 80°C in the absence of substrate. Calcium is not required for thermostability. Cu2+, Hg2+ and EDTA inhibit the protease activity while Fe2+ and Fe3+ ions enhanced the activity. SDS-PAGE studies indicate the presence of multiple protease activity bands with molecular masses between 40 and 57 kDa. The proteases show higher activity towards short synthetic peptides while a decrease in specificity was observed with longer peptides. en_US
dc.language.iso en en_US
dc.title Production and properties of thermostable proteases from bacillus thermoleovorans strain HSR en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199736770 en_US
dc.author.idnumber 199390070 en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal Scientifique Libanais en_US
dc.journal.volume 1 en_US
dc.journal.issue 1 en_US
dc.article.pages 49-61 en_US
dc.keywords Bacillus thermoleovorans en_US
dc.keywords Proteolytic en_US
dc.keywords Protease en_US
dc.keywords Thermophilic en_US
dc.keywords Thermostability en_US
dc.identifier.ctation Tokajian, S., Sunna, A., & Hashwa, F. (2000). PRODUCTION AND PROPERTIES OF THERMOSTABLE PROTEASES FROM BACILLUS THERMOLEOVORANS STRAIN HSR. Journal Scientifique Libanais, 1(1), 49. en_US
dc.author.email stokjian@lau.edu.lb en_US
dc.author.email fhashwa@lau.edu.lb
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://lsj.cnrs.edu.lb/wp-content/uploads/2016/01/hashwa.pdf en_US
dc.orcid.id https://orcid.org/0000-0002-3653-8940
dc.author.affiliation Lebanese American University en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search LAUR

Advanced Search


My Account