Characterization of streptococcus pyogenes isolates from Lebanon. (c2012)

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dc.contributor.author Karaky, Nathalie Mahmoud
dc.date.accessioned 2017-01-25T13:52:02Z
dc.date.available 2017-01-25T13:52:02Z
dc.date.copyright 2012 en_US
dc.date.issued 2017-01-25
dc.date.submitted 2012-06-07
dc.identifier.uri http://hdl.handle.net/10725/5067
dc.description.abstract Streptococcus pyogenes (Group A Streptococcus [GAS]) is one of the clinically most important human pathogens responsible for numerous diseases with diverse clinical manifestations. GAS infections range from mild superficial skin infections to life-threatening systemic diseases associated with high morbidity and mortality rates. As the epidemiology of GAS infections changes, a rapid and trustworthy characterization of the isolates is required for epidemiological analysis and infection control. This study aimed at the molecular characterization of GAS isolated from Lebanon. Isolates were characterized by emm typing, superantigen and antibiotic profiles. Fourty-one distinct emm types were detected among the 150 isolates, the most prevalent of which were emm types: 89 (16%), 12 (10%), 2 (9%) and 1 (8%), previously associated with invasive infections. Five isolates were non-typeable. Antibiotic susceptibility testing against seven different classes revealed that all were susceptible to vancomycin, penicillin G and ampicillin, 9% were resistant to clindamycin, 23% were resistant to erythromycin and only 4% were resistant to erythromycin and clindamycin, showing the macrolide–lincosamide–streptogramin B phenotype. An unexpected high emergence of tetracycline resistant strains (37%) was noted and hence our results confirm that resistance of S. pyogenes is a significant problem in Lebanon. Testing for superantigen showed that SpeB (87%) followed by ssa (36%) and SpeG (30%) were the predominant superantigens recovered, but no clear conclusion was excluded from this study concerning the role of superantigens in disease manifestation. This study provides a database of emm sequences, resistance patterns and superantigen profiles of S. pyogenes, which will assist in long-term epidemiological and evolutionary studies of this significant pathogen in Lebanon. Further surveillance studies are required to understand the local epidemiology of GAS in Lebanon. en_US
dc.language.iso en en_US
dc.subject Streptococcus pyogenes -- Lebanon en_US
dc.subject Streptococcal infections -- Lebanon en_US
dc.subject Molecular epidemiology en_US
dc.subject Dissertations, Academic en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.title Characterization of streptococcus pyogenes isolates from Lebanon. (c2012) en_US
dc.type Thesis en_US
dc.title.subtitle pathogenesis, epidemiology and resistance en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 200904780 en_US
dc.author.commembers Khalaf, Roy
dc.author.commembers Wex, Brigitte
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xv, 56 leaves; col., ill.; 31 cm. available at RNL. en_US
dc.author.advisor Tokajian, Sima
dc.keywords Streptococcus pyogenes en_US
dc.keywords GAS en_US
dc.keywords emm typing en_US
dc.keywords Superantigens en_US
dc.keywords Antibiotics en_US
dc.description.bibliographiccitations Includes bibliographical references (leaves 35-52). en_US
dc.identifier.doi https://doi.org/10.26756/th.2012.45 en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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