The crosstalk between RhoC and Cdc42 during invadopodia formation mediated by Palladin. (c2016)

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dc.contributor.author Merheb, Emilio C.
dc.date.accessioned 2016-12-09T09:42:41Z
dc.date.available 2016-12-09T09:42:41Z
dc.date.copyright 2016 en_US
dc.date.issued 2016-12-09
dc.date.submitted 2016-04-27
dc.identifier.uri http://hdl.handle.net/10725/4904
dc.description.abstract Cell metastasis is the main difference between malignant and benign tumors, and it is a main contributor to the high mortality rate of malignant cancer. Therefore, it is of great interest to study the pathways involved in the motility of cancer cells, which are regulated by the Rho family of small GTPases. This study examines the role of the Rho GTPases, RhoC and Cdc42 in 3D cell motility, mainly in the formation of actin structures termed invadopodia. RhoC was shown to play a role in both 2D and 3D cell motility; whereas, the action of Cdc42 is limited to 3D motility. We first established the formation of invadopodia through PMA stimulation. Next, our results showed that these invadopodia are Cdc42 dependent and that the inhibition of ROCK leads to the increase in invadopodia formation. It was also shown that RhoC downregulates the activation of Cdc42 which leads to the final conclusion that RhoC is active around the invadopodia structure, where it inhibits Cdc42 through the action of ROCK, allowing the invadopodia structure to become more concentrated and focused. Additionally, Palladin protein was shown to inhibit Cdc42, which may imply a crosstalk between palladin, RhoC and Cdc42 in invadopodia formation. en_US
dc.language.iso en en_US
dc.subject Cancer cells -- Motility en_US
dc.subject Rho GTPases en_US
dc.subject Metastasis en_US
dc.subject Dissertations, Academic en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.title The crosstalk between RhoC and Cdc42 during invadopodia formation mediated by Palladin. (c2016) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201001595 en_US
dc.author.commembers Abi Habib, Ralph
dc.author.commembers Khalaf, Roy
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xi, 51 leaves; ill.; 31 cm. available at RNL. en_US
dc.author.advisor El-Sibai, Mirvat
dc.keywords Invadopodia en_US
dc.keywords Cell Motility en_US
dc.keywords RhoC en_US
dc.keywords Cdc42 en_US
dc.keywords ROCK en_US
dc.keywords Palladin en_US
dc.description.bibliographiccitations Bibliography : leaves 45-51. en_US
dc.identifier.doi https://doi.org/10.26756/th.2016.30 en_US
dc.author.email emilio.merheb@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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