A Novel Role Of GATA6 in Rhabdomyosarcoma. (c2016)

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dc.contributor.author Khoury, Michelle Fady El
dc.date.accessioned 2016-12-08T11:31:53Z
dc.date.available 2016-12-08T11:31:53Z
dc.date.copyright 2016 en_US
dc.date.issued 2016-12-08
dc.date.submitted 2016-05-04
dc.identifier.uri http://hdl.handle.net/10725/4902
dc.description.abstract One of the major events during embryogenesis is skeletal muscle formation. Over the years, this developmental process has been of great interest to many scientists. There’s no cancer in skeletal muscles. However, some cells are known to express differentiation markers such as Myf5 and MyoD; these are the Rhabdomyosarcoma cells (RH30 and JR1). They harbor skeletal muscle characteristics, express early markers of differentiation but fail however in forming myotubes. The movement of cells from the proliferation to the differentiation stage is controlled by a specific group of myogenic factors. Deregulation of these transcription factors result in muscular defects and skeletal muscle diseases. GATA6 is one of the myogenic factors that was found to be expressed in rhabdomyosarcoma cell lines and might play a role in down-regulating cellular proliferation and causing cell death. The objective of this study is to understand the role of GATA6 in Rhabdomyosarcoma by assessing its expression at the transcriptional level and studying the effect of its down-regulating, by specific siRNA, on cell cycle and cellular proliferation. Real time PCR was used to assess the expression of GATA6 in Rhabdomyosarcoma cell lines and Flow Cytometry was used to study the effect of inhibition on the cell cycle. GATA6 was shown to be expressed at the proliferation and differentiation stage. Down-regulation of GATA6 by siRNA reduced the cellular proliferative potential and caused cell death. We conclude that the down-regulation of GATA6 could represent a potential venue to develop drugs against rhabdomyosarcoma. en_US
dc.language.iso en en_US
dc.subject Rhabdomyosarcoma en_US
dc.subject Muscles -- Tumors en_US
dc.subject Flow cytometry en_US
dc.subject Dissertations, Academic en_US
dc.subject Lebanese American University -- Dissertations en_US
dc.title A Novel Role Of GATA6 in Rhabdomyosarcoma. (c2016) en_US
dc.type Thesis en_US
dc.term.submitted Spring en_US
dc.author.degree MS in Molecular Biology en_US
dc.author.school SAS en_US
dc.author.idnumber 201204743 en_US
dc.author.commembers El Sibai, Mirvat
dc.author.commembers Ralph, Abi Habib
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.description.physdesc 1 hard copy: xi, 28 leaves; 31 cm. available at RNL. en_US
dc.author.advisor Tokajian, Sima
dc.keywords GATA6 en_US
dc.keywords Rhabdomyosarcoma en_US
dc.keywords SiRNA en_US
dc.keywords Real-Time PCR en_US
dc.keywords Flow Cytometry en_US
dc.description.bibliographiccitations Bibliography : leaves 25-28. en_US
dc.identifier.doi https://doi.org/10.26756/th.2016.28 en_US
dc.author.email michelle.elkhoury@lau.edu en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/thesis.php en_US
dc.publisher.institution Lebanese American University en_US
dc.author.affiliation Lebanese American University en_US

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