C1 neurons excite locus coeruleus and A5 noradrenergic neurons along with sympathetic outflow in rats

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dc.contributor.author Abbott, S. B.
dc.contributor.author Kanbar, R.
dc.contributor.author Bochorishvili, G.
dc.contributor.author Coates, M. B.
dc.contributor.author Stornetta, R. L.
dc.contributor.author Guyenet, P. G. en_US
dc.date.accessioned 2016-10-11T06:24:50Z
dc.date.available 2016-10-11T06:24:50Z
dc.date.copyright 2012 en_US
dc.date.issued 2016-10-11
dc.identifier.issn 0022-3751 en_US
dc.identifier.uri http://hdl.handle.net/10725/4556
dc.description.abstract C1 neurons activate sympathetic tone and stimulate the hypothalamic–pituitary–adrenal axis in circumstances such as pain, hypoxia or hypotension. They also innervatepontine noradrenergic cell groups, including the locus coeruleus (LC) and A5. Activation ofC1 neurons reportedly inhibits LC neurons; however, because these neurons are glutamatergicand have excitatory effects elsewhere, we re-examined the effect of C1 activation on pontinenoradrenergic neurons (LC and A5) using a more selective method. Using a lentivir us thatexpresses channelrhodopsin2 (ChR2) under the control of the artificial promoter PRSx8, werestricted ChR2 expression to C1 neurons (67%), retrotrapezoid nucleus neurons (20%) andcholinergic neurons (13%). The LC contained ChR2-positive terminals that formed asymmetricsynapses and were immunoreactive for vesicular glutamate transporter type 2. Low-frequencyphotostimulation of ChR2-expressing neurons ac tivated LC (38 of 65; 58%) and A5 neurons (11 of16; 69%) and sympathetic ner ve discharge. Locus coeruleus and A5 inhibition was not seen unlesspreceded by excitation. Locus coeruleus activation was eliminated by intracerebroventricularkynurenic acid. Stimulation of ChR2-expressing neurons at 20 Hz produced modest increasesin LC and A5 neuronal discharge. In additional rats, the retrotrap ezoid nucleus region wasdestroyed with substance P–saporin prior to lentivirus injection into the rostral ventrolateralmedulla, increasing the propor tion of C1 ChR2-expressing neurons (83%). Photostimulation inthese rats activated the same proportion of LC and A5 neurons as in control rats but producedno effect on sympathetic nerve discharge owing to the destruction of bulbospinal C1 neurons. Inconclusion, low-frequency stimulation of C1 n eurons activates pontine noradrenergic neurons. en_US
dc.language.iso en en_US
dc.title C1 neurons excite locus coeruleus and A5 noradrenergic neurons along with sympathetic outflow in rats en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOP en_US
dc.author.idnumber 201005298 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of Physiology en_US
dc.journal.volume 590 en_US
dc.journal.issue 12 en_US
dc.article.pages 2897-2915 en_US
dc.identifier.doi http://dx.doi.org/10.1113/jphysiol.2012.232157 en_US
dc.identifier.ctation Abbott, S. B., Kanbar, R., Bochorishvili, G., Coates, M. B., Stornetta, R. L., & Guyenet, P. G. (2012). C1 neurons excite locus coeruleus and A5 noradrenergic neurons along with sympathetic outflow in rats. The Journal of physiology, 590(12), 2897-2915. en_US
dc.author.email roy.kanbar@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url https://physoc.onlinelibrary.wiley.com/doi/full/10.1113/jphysiol.2012.232157 en_US
dc.orcid.id https://orcid.org/0000-0001-5450-6443 en_US

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