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Regulation of visceral sympathetic tone by A5 noradrenergic neurons in rodents

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dc.contributor.author Kanbar, Roy
dc.contributor.author Depuy, Gavin H.
dc.contributor.author West, Ruth L.
dc.contributor.author Guyenet, Stornetta and Patrice G.
dc.date.accessioned 2016-10-10T11:33:52Z
dc.date.available 2016-10-10T11:33:52Z
dc.date.copyright 2011 en_US
dc.identifier.issn 0928-4257 en_US
dc.identifier.uri http://hdl.handle.net/10725/4550
dc.description.abstract The ventrolateral pons contains the A5 group of noradrenergic neurons which regulate the circulation and probably breathing. The present experiments were designed to identify these neurons definitively in vivo, to examine their response to chemoreceptor stimuli (carotid body stimulation and changes in brain pH) and to determine their effects on sympathetic outflow. Bulbospinal A5 neurons, identified by juxtacellular labelling in anaesthetized rats, had a slow regular discharge, were vigorously activated by peripheral chemoreceptor stimulation with cyanide, but only mildly activated by hyperoxic hypercapnia (central chemoreceptor stimulation). The caudal end of the A5 region also contained neurons with properties reminiscent of retrotrapezoid neurons. These cells lacked a spinal axon and were characterized by a robust response to CO2. The pH sensitivity of A5 neurons, examined in brain slices from neonatal (postnatal days 6–10) tyrosine hydroxylase (TH)-GFP transgenic mice, was about 10 times smaller than that of similarly recorded retrotrapezoid neurons. Selective stimulation of the A5 neurons in rats using channelrhodopsin optogenetics (A5 TH neurons represented 66% of transfected cells) produced fivefold greater activation of the renal nerve than the lumbar sympathetic chain. In summary, adult A5 noradrenergic neurons are vigorously activated by carotid body stimulation. This effect presumably contributes to the increase in visceral sympathetic nerve activity elicited by acute hypoxia. A5 neurons respond weakly to hypercapnia in vivo or to changes in pH in slices suggesting that their ability to sense local variations in brain pH or PCO2 is limited. en_US
dc.language.iso en en_US
dc.title Regulation of visceral sympathetic tone by A5 noradrenergic neurons in rodents en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SOP en_US
dc.author.idnumber 201005298 en_US
dc.author.department N/A en_US
dc.description.embargo N/A en_US
dc.relation.journal Journal of physiology en_US
dc.journal.volume 589 en_US
dc.journal.issue 4 en_US
dc.article.pages 903-917 en_US
dc.identifier.doi http://dx.doi.org/10.1006/bbrc.1994.188310.1113/jphysiol.2010.198374 en_US
dc.identifier.ctation Kanbar, R., Depuy, S. D., West, G. H., Stornetta, R. L., & Guyenet, P. G. (2011). Regulation of visceral sympathetic tone by A5 noradrenergic neurons in rodents. The Journal of physiology, 589(4), 903-917. en_US
dc.author.email roy.kanbar@lau.edu.lb en_US
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1113/jphysiol.2010.198374/full en_US


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