Buccal Cell DNA Extraction

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dc.contributor.author Saab, Yolande B.
dc.contributor.author Kabbara, Wissam
dc.contributor.author Chebib, Christiane
dc.contributor.author Gard, Paul Richard
dc.date.accessioned 2016-09-26T08:45:38Z
dc.date.available 2016-09-26T08:45:38Z
dc.date.copyright 2008 en_US
dc.date.issued 2016-09-26
dc.identifier.issn 1090-6576 en_US
dc.identifier.uri http://hdl.handle.net/10725/4382
dc.description.abstract Simple and cost-effective methods are needed to extract DNA in order to use it in large-scale studies. Blood is an excellent DNA source; however, it is costly and invasive thus an alternative is needed. Several kits and chemical protocols using buccal cells have been proposed for DNA extraction. The objective of the study is to evaluate buccal NaOH chemical protocol and Nucleospin® Tissue Kit (BD Biosciences, Macery-Nagel, Germany) for DNA extraction. DNA swab samples were collected from 300 voluntary participants. DNA yields and purity were measured by NaOH and Nucleospin® Tissue Kit techniques; the cost and time consumption for DNA extraction per sample were assessed as well. Results have shown that DNA amount and purity extracted by NaOH procedure was compared to that of the kit (p = 0.164; p = 0.249, respectively). NaOH method was considered cheaper and less time consuming (0.06 versus 3.80 USD, and 1.33 versus 3.59 minutes per sample, p < 0.001). Buccal cell derived DNA extracted by NaOH protocol can be considered a feasible substitute for more expensive and time-consuming kits. en_US
dc.language.iso en en_US
dc.title Buccal Cell DNA Extraction en_US
dc.type Article en_US
dc.description.version Published en_US
dc.title.subtitle yield, purity, and cost: a comparison of two methods en_US
dc.author.school SOP en_US
dc.author.idnumber 199110360 en_US
dc.author.idnumber 200104485
dc.author.department Pharmacy Practice en_US
dc.description.embargo N/A en_US
dc.relation.journal Genetic Testing en_US
dc.journal.volume 11 en_US
dc.journal.issue 4 en_US
dc.article.pages 413-416 en_US
dc.identifier.doi http://dx.doi.org/10.1089/gte.2007.0044 en_US
dc.identifier.ctation Saab, Y. B., Kabbara, W., Chbib, C., & Gard, P. R. (2007). Buccal cell DNA extraction: yield, purity, and cost: a comparison of two methods. Genetic Testing, 11(4), 413-416. en_US
dc.author.email ysaab@lau.edu.lb en_US
dc.author.email wissam.kabbara@lau.edu.lb
dc.identifier.tou http://libraries.lau.edu.lb/research/laur/terms-of-use/articles.php en_US
dc.identifier.url http://online.liebertpub.com/doi/abs/10.1089/gte.2007.0044 en_US
dc.orcid.id https://orcid.org/0000-0002-7310-9192 en_US

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