dc.contributor.author |
Na'was, Tarek |
|
dc.contributor.author |
Essawi, Tamer |
|
dc.contributor.author |
Hawwari, Abbass |
|
dc.contributor.author |
Wadi, Suzan |
|
dc.contributor.author |
Doudin, Anwar |
|
dc.contributor.author |
Fattom, Ali Ibrahim |
|
dc.date.accessioned |
2016-04-14T13:49:20Z |
|
dc.date.available |
2016-04-14T13:49:20Z |
|
dc.date.copyright |
2002 |
|
dc.date.issued |
2016-04-14 |
|
dc.identifier.issn |
1360-2276 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/10725/3565 |
|
dc.description.abstract |
Staphylococcus aureus is a major cause of nosocomial infections and a risk in patients who have either undergone surgery or are on haemodialysis. The S. aureus infections in patients admitted to the clinical departments of Al-Makased Charitable Hospital in Jerusalem during a period of one year were investigated. Isolates included were from blood, surgical wounds, or other nonsuperficial sites. Of 63 isolates available for analysis, 46 (73.0%) expressed type 8 capsular polysaccharide; 13 (20.7%), type 5 capsular polysaccharide; only 4 isolates (6.3%) did not express type 5 or type 8 antibodies. The strains fitted in 7 different antibiogram types, with the type showing resistance only to penicillin and ampicillin prevalent in 34 out of 63 isolates (54.0%). Of the 12 methicillin-resistant S. aureus (MRSA) isolates (19.1%), 8(66.7%) possessed the type 8 capsule and 4(33.7%) the type 5 capsule. Pulsed-field gel electrophoresis of all isolates with the restriction-endonuclease enzymes Sma I revealed 34 patterns demonstrating that no single methicillin-sensitive S. aureus strain was endemic in the hospital. However, all MRSA isolates with a type 8 capsule showed identical PFGE patterns using the 2 restriction-endonuclease enzymes Sma I and SST II. Moreover, type 5 isolates showed identical patterns (one isolate differed from the rest with one band only). These data suggest and confirm the clonality of type 5 and type 8 MRSA isolates. Analysing the results of the capsular and antibiogram typing schemes in conjunction proved useful and suggested that such an analysis can be employed as a helpful epidemiological tool in hospitals with limited resources. |
en_US |
dc.language.iso |
en |
en_US |
dc.title |
Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem |
en_US |
dc.type |
Article |
en_US |
dc.description.version |
Published |
en_US |
dc.author.school |
SAS |
en_US |
dc.author.idnumber |
198029370 |
|
dc.author.woa |
N/A |
en_US |
dc.author.department |
Natural Sciences |
en_US |
dc.description.embargo |
N/A |
en_US |
dc.relation.journal |
Tropical Medicine & International Health |
en_US |
dc.journal.volume |
3 |
en_US |
dc.journal.issue |
7 |
en_US |
dc.article.pages |
576-583 |
en_US |
dc.keywords |
Staphylococcus aureu |
en_US |
dc.keywords |
Nosocomial infection |
en_US |
dc.keywords |
Epidemiology |
en_US |
dc.keywords |
Pulsed-field-gel electrophoresis |
en_US |
dc.identifier.doi |
http://dx.doi.org/10.1046/j.1365-3156.1998.00265.x |
en_US |
dc.identifier.ctation |
Essawi, T., Na'was, T., Hawwari, A., Wadi, S., Doudin, A., & Fattom, A. I. (1998). Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al‐Makased hospital in East Jerusalem. Tropical Medicine & International Health, 3(7), 576-583. |
en_US |
dc.author.email |
tnawas@lau.edu.lb |
|
dc.identifier.url |
http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3156.1998.00265.x/full |
|