Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem

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dc.contributor.author Na'was, Tarek
dc.contributor.author Essawi, Tamer
dc.contributor.author Hawwari, Abbass
dc.contributor.author Wadi, Suzan
dc.contributor.author Doudin, Anwar
dc.contributor.author Fattom, Ali Ibrahim
dc.date.accessioned 2016-04-14T13:49:20Z
dc.date.available 2016-04-14T13:49:20Z
dc.date.copyright 2002
dc.date.issued 2016-04-14
dc.identifier.issn 1360-2276 en_US
dc.identifier.uri http://hdl.handle.net/10725/3565
dc.description.abstract Staphylococcus aureus is a major cause of nosocomial infections and a risk in patients who have either undergone surgery or are on haemodialysis. The S. aureus infections in patients admitted to the clinical departments of Al-Makased Charitable Hospital in Jerusalem during a period of one year were investigated. Isolates included were from blood, surgical wounds, or other nonsuperficial sites. Of 63 isolates available for analysis, 46 (73.0%) expressed type 8 capsular polysaccharide; 13 (20.7%), type 5 capsular polysaccharide; only 4 isolates (6.3%) did not express type 5 or type 8 antibodies. The strains fitted in 7 different antibiogram types, with the type showing resistance only to penicillin and ampicillin prevalent in 34 out of 63 isolates (54.0%). Of the 12 methicillin-resistant S. aureus (MRSA) isolates (19.1%), 8(66.7%) possessed the type 8 capsule and 4(33.7%) the type 5 capsule. Pulsed-field gel electrophoresis of all isolates with the restriction-endonuclease enzymes Sma I revealed 34 patterns demonstrating that no single methicillin-sensitive S. aureus strain was endemic in the hospital. However, all MRSA isolates with a type 8 capsule showed identical PFGE patterns using the 2 restriction-endonuclease enzymes Sma I and SST II. Moreover, type 5 isolates showed identical patterns (one isolate differed from the rest with one band only). These data suggest and confirm the clonality of type 5 and type 8 MRSA isolates. Analysing the results of the capsular and antibiogram typing schemes in conjunction proved useful and suggested that such an analysis can be employed as a helpful epidemiological tool in hospitals with limited resources. en_US
dc.language.iso en en_US
dc.title Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 198029370
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Tropical Medicine & International Health en_US
dc.journal.volume 3 en_US
dc.journal.issue 7 en_US
dc.article.pages 576-583 en_US
dc.keywords Staphylococcus aureu en_US
dc.keywords Nosocomial infection en_US
dc.keywords Epidemiology en_US
dc.keywords Pulsed-field-gel electrophoresis en_US
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-3156.1998.00265.x en_US
dc.identifier.ctation Essawi, T., Na'was, T., Hawwari, A., Wadi, S., Doudin, A., & Fattom, A. I. (1998). Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al‐Makased hospital in East Jerusalem. Tropical Medicine & International Health, 3(7), 576-583. en_US
dc.author.email tnawas@lau.edu.lb
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3156.1998.00265.x/full

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