Toxins and Antibiotic Resistance in Staphylococcus aureus Isolated from a Major Hospital in Lebanon

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dc.contributor.author Tokajian, Sima
dc.contributor.author Hashwa, Fuad
dc.contributor.author Andraos, Rana
dc.contributor.author Haddad, Dominik
dc.contributor.author Araj, George
dc.date.accessioned 2016-03-23T12:32:10Z
dc.date.available 2016-03-23T12:32:10Z
dc.date.copyright 2011
dc.date.issued 2016-03-23
dc.identifier.issn 2090-7478 en_US
dc.identifier.uri http://hdl.handle.net/10725/3401
dc.description.abstract Molecular characterization of Staphylococcus aureus is of both clinical and infection control importance. Virulence determinants using PCR and multiple drug resistance profiles were studied in 130 S. aureus isolates. PCR-RFLP analysis of the 16S–23S DNA spacer region was done to investigate the level of 16S–23S ITS (internal transcribed spacer) polymorphism. Methicillin-resistant S. aureus (MRSA), which represented 72% of the studied isolates, showed multiple drug resistance with 18% being resistant to 10–18 of the drugs used compared to a maximum resistance to 9 antibiotics with the methicillin sensitive S. aureus (MSSA) isolates. Exfoliative toxin A (ETA) was more prevalent than B (ETB) with virulent determinants being additionally detected in multiple drug-resistant isolates. 16S–23S ITS PCR-RFLP combined with sequencing of the primary product was successful in generating molecular fingerprints of S. aureus and could be used for preliminary typing. This is the first study to demonstrate the incidence of virulent genes, ACME, and genetic diversity of S. aureus isolates in Lebanon. The data presented here epitomize a starting point defining the major genetic populations of both MRSA and MSSA in Lebanon and provide a basis for clinical epidemiological studies en_US
dc.language.iso en en_US
dc.title Toxins and Antibiotic Resistance in Staphylococcus aureus Isolated from a Major Hospital in Lebanon en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199390070 en_US
dc.author.idnumber 199736770 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal ISRN Microbiology en_US
dc.journal.volume 2011 en_US
dc.article.pages 1-10 en_US
dc.identifier.doi http://dx.doi.org/10.5402/2011/812049 en_US
dc.identifier.ctation Tokajian, S., Haddad, D., Andraos, R., Hashwa, F., & Araj, G. (2011). Toxins and antibiotic resistance in Staphylococcus aureus isolated from a major hospital in Lebanon. ISRN microbiology, 2011. en_US
dc.author.email stokjian@lau.edu.lb
dc.author.email fhashwa@lau.edu.lb
dc.identifier.url https://www.researchgate.net/profile/Fuad_Hashwa/publication/236978868_Toxins_and_Antibiotic_Resistance_in_Staphylococcus_aureus_Isolated_from_a_Major_Hospital_in_Lebanon/links/00b7d528eec63cba01000000.pdf

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