Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks

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dc.contributor.author Tokajian, Sima
dc.contributor.author Hashwa, Fuad
dc.date.accessioned 2016-03-23T11:59:57Z
dc.date.available 2016-03-23T11:59:57Z
dc.date.copyright 2004
dc.date.issued 2016-03-23
dc.identifier.issn 1201-3080 en_US
dc.identifier.uri http://hdl.handle.net/10725/3399
dc.description.abstract Upon storage, a statistically significant increase in heterotrophic plate count (HPC) was detected in samples from household tanks. The most consistent correlate of regrowth was found to be storage time, however, temperature and the microbiological quality of the influent water were also important. Total and fecal coliforms were detected in the influent water and in the household storage tanks. Coliforms growing at 37ºC were a small but significant part (about 0.002%) of the total heterotrophic population. In the influent, 92% of the coliforms (11 colony forming units/100 mL) at 44ºC were Escherichia coli, while in the household tanks it was 86%. The majority of isolates from the influent and the household tanks recovered on R2A agar formed pigmented colonies and were Gram-negative belonging to the α-, β-, and γ-subclasses of Proteobacteria. Overall, the α-subclass was the dominant type representing 61% of the recovered HPC, and 40 to 50% of the organisms based on 16S rDNA sequence analysis were members of the family Sphingomonadaceae. The presence of E. coli and the isolation of opportunistic pathogens as a significant part of the HPC population from both influent and household tanks indicated a potential health hazard to consumers. en_US
dc.language.iso en en_US
dc.title Microbiological Quality and Genotypic Speciation of Heterotrophic Bacteria Isolated from Potable Water Stored in Household Tanks en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199390070 en_US
dc.author.idnumber 199736770 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Water Quality Research Journal of Canada en_US
dc.journal.volume 39 en_US
dc.journal.issue 1 en_US
dc.article.pages 64-73 en_US
dc.keywords Household en_US
dc.keywords Storage en_US
dc.keywords Escherichia coli en_US
dc.keywords 16S rDNA en_US
dc.keywords Sphingomonas en_US
dc.keywords Aeromonas en_US
dc.identifier.ctation Tokajian, S., & Hashwa, F. (2004). Microbiological quality and genotypic speciation of heterotrophic bacteria isolated from potable water stored in household tanks. Water quality research journal of Canada, 39(1), 64-73. en_US
dc.author.email stokjian@lau.edu.lb
dc.author.email fhashwa@lau.edu.lb
dc.identifier.url https://www.cawq.ca/journal/temp/journal/3.pdf#page=70
dc.orcid.id https://orcid.org/0000-0002-3653-8940

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