The reductive enzymatic cleavage of thiosulfate

LAUR Repository

Show simple item record

dc.contributor.author Hashwa, Fuad
dc.contributor.author Pfennig, Norbert
dc.date.accessioned 2016-03-23T10:32:55Z
dc.date.available 2016-03-23T10:32:55Z
dc.date.copyright 1972
dc.date.issued 2016-03-23
dc.identifier.issn 0003-9276 en_US
dc.identifier.uri http://hdl.handle.net/10725/3393
dc.description.abstract Methods presently used for the enzymatic assay of the thiosulfate cleaving reaction in bacterial cell-free systems are critically examined. Conditions under which strong acids are used to terminate the reaction and to release H2S proved to be unsuitable. A non-enzymatic production of H2S under such conditions is demonstrated. A reliable procedure for the measurement of H2S production from the enzymatic cleavage of thiosulfate is described. This method was used to measure the thiosulfate cleaving reaction catalyzed by cell-free extracts of phototrophic bacteria of the genusChromatium. As reductants, hydrogen-hydrogenase/methyl viologen system, reduced glutathione or dihydrolipoate were used. The same extract fraction catalyzed the rhodanese reaction. en_US
dc.language.iso en en_US
dc.title The reductive enzymatic cleavage of thiosulfate en_US
dc.type Article en_US
dc.description.version Published en_US
dc.author.school SAS en_US
dc.author.idnumber 199390070 en_US
dc.author.woa N/A en_US
dc.author.department Natural Sciences en_US
dc.description.embargo N/A en_US
dc.relation.journal Archiv für Mikrobiologie en_US
dc.journal.volume 81 en_US
dc.journal.issue 1 en_US
dc.article.pages 36-44 en_US
dc.identifier.doi http://dx.doi.org/10.1007/BF00715022 en_US
dc.identifier.ctation Hashwa, F., & Pfennig, N. (1972). The reductive enzymatic cleavage of thiosulfate. Archiv für Mikrobiologie, 81(1), 36-44. en_US
dc.author.email fhashwa@lau.edu.lb
dc.identifier.url http://link.springer.com/article/10.1007/BF00715022

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search LAUR

Advanced Search


My Account